TEMPLATE POLYMERIZATION

分子印迹是在交联网络聚合物中为有机分子创造结合和受体位点的一种通用方法。 该提案的目标是扩展分子印迹的边界，以创建药物和药物中间体，寡核苷酸和其他复杂有机靶标的人工受体和结合位点。具体来说，我们建议拓宽分子印迹可以靶向的分子范围。 我们将开发用于印迹酸性化合物和含有全氟烷基的分子的新配方。 我们还建议合成苯二氮卓类受体、寡核苷酸、碳水化合物衍生物、小肽和药物合成中间体。将印迹聚合物整合到有用的技术中需要能够以各种形态制造这些材料。 沿着这些路线，我们已经开发了分子印迹薄膜和膜（MIM）的方法。 我们建议评估这些材料的一些应用，包括它们作为聚合物相在固相萃取装置中的使用，并作为半透膜分离复杂的有机分子和对映体。压印薄膜也将作为传感器设备的识别元件进行评估。 后一种应用将利用荧光信号来报告结合事件。 最后，我们提出了一项研究，旨在了解在聚合过程中如何创建结合位点。

Molecular imprinting is a general method for creating binding and receptor sites for organic molecules in crosslinked network polymers. The objectives of this proposal are to expand the boundaries of molecular imprinting to create artificial receptors and binding sites for drugs and drug intermediates, oligonucleotides, and other complex organic targets. Specifically we propose to broaden the range of molecules that can be targeted by molecular imprinting. We will develop new formulations for imprinting acidic compounds and molecules that contain perfluoroalkyl groups. We also propose to synthesize receptors for benzodiazepines, oligonucleotides, carbohydrate derivatives, small peptides, and intermediates in drug synthesis. The integration of imprinted polymers into useful technology requires an ability to fabricate these materials in a variety of morphologies. Along these lines we have developed methods for molecularly imprinted thin films and membranes (MIM's). We propose to evaluate these materials for a number of applications, including their use as the polymer phase in solid phase extraction devices, and as semipermeable membranes for the separation of complex organic molecules and enantiomers. Imprinted films will also be evaluated as the recognition element for sensor devices. This latter application will utilize a fluorescent signal to report the binding event. Finally we have proposed a study designed to get at how binding sites are created during the polymerization process.