MASS SPECTROMETRY OF METAL COORDINATED OLIGOSACCHARIDES

该项目的长期目标是完成结构鉴定。 利用串联质谱仪对金属配位的寡糖进行分析。 本文提出的主要领域包括：1)微波离子陷阱 立体异构体在线分析研究进展2)新型化合物 合成：用于多种金属研究的模板，3)分化和 立体异构体糖的定量，4)水加成物 金属/配体/低聚糖：气相结构研究， 5)生物分离低聚糖的表征 金属配位方法论和6)计算研究。它是 预计所有功能化的立体化学分析 糖类可以通过我们的金属/配体衍生化来实现。 方法。微波离子陷阱技术领域的进步已经 允许100 ng糖的在线合成和分析 不到五分钟。隔离方法的发展与分析 化合物将通过标记研究和理论研究完成 为了预测更好的金属和配体并获得 关于解离机理、金属配位位置的信息 以及去质子化的地点。

The long term goal of this project is complete structural identification of metal coordinated oligosaccharides using tandem mass spectrometry. The major areas proposed herein include 1) Microwave-ion trap development for on-line analysis of stereoisomers, 2) Novel compound synthesis: Template for multiple metal studies, 3) Differentiation and quantification of stereoisomeric saccharides, 4) Water adducts of metal/ligand/oligosaccharides: Investigations into gas phase structure, 5) Characterization of biologically isolated oligosaccharides using metal-coordination methodology, and 6) Computational studies. It is anticipated that stereochemical analysis of all functionalized saccharides can be achieved using our metal/ligand derivatization method. Advancements in the area of microwave-ion trap technology has allowed the synthesis and analysis of 100 ng of saccharide on line in less than five minutes. Methods development and analysis of isolated compounds will be completed with labeling studies and theoretical calculations in order to predict better metals and ligands and to obtain information on mechanisms of dissociation, site of metal coordination and sites of deprotonation.