INTEGRATED MAPS USING RADIATION HYBRIDS

描述：在这个R29应用程序中，调查者建议开发 并改进计算工具以分析从辐射获得的结果 杂交和连锁作图，特别强调整合 多张地图。调查员建议使用各种最大值 确定辐射杂波积分最佳方法的似然方法 从不同的辐射混合体收集数据的地图 面板，以及将辐射混合地图数据与链接地图集成 数据。她将使用几个小组产生的数据，这些小组已经建立了 具有三种不同混合面板的辐射混合地图，旨在允许 待确定的分辨率范围(从几Mb到50 kb)，数据来自 几个广泛的链接地图，以及来自YAC地图的数据，其中 可以估计距离信息以允许碱基对的比较， 厘米摩根和厘米射线距离待定。另一个重要的部分 这项工作将提高构建辐射混合地图的能力 通过修改多地图计算机来获得非常大量的标记 程序，该程序由调查者开发，用于连锁作图。她 也将使用这些工具来参与最近开始的小鼠基因组 辐射混合测绘项目。最后，她建议发展 基于Web的服务器，允许外部用户计算地图位置 在多个公共辐射杂交和连锁图上，标记或一组 记号笔。

DESCRIPTION: In this R29 application, the investigator proposes to develop and refine computational tools for analyzing results obtained from radiation hybrid and linkage mapping, with a particular emphasis on integration of multiple maps. The investigator proposes to use a variety of maximum likelihood methods to determine the best way to integrate radiation hybrid maps in which data have been gathered from different radiation hybrid panels, as well as to integrate radiation hybrid map data with linkage map data. She will use data generated by several groups who have built radiation hybrid maps with three different hybrid panels designed to allow a range of resolutions (from several Mb to 50 kb) to be determined, data from several extensive linkage maps, and data from YAC maps where physical distance information can be estimated to allow comparisons of base pair, centiMorgan and centiRay distances to be made. Another significant part of this effort will be to improve the ability to build radiation hybrid maps with very large numbers of markers by modifying the MultiMap computer program, which was developed by the investigator for linkage mapping. She will also use these tools to participate in a recently begun mouse genome radiation hybrid mapping project. Finally, she proposes to develop web-based servers to allow outside users to calculate map positions, based on multiple public radiation hybrid and linkage maps, of a marker or set of markers.