VOLTAGE GATED ION CHANNELS--STRUCTURE AND EXPRESSION

描述：这是一个更新的应用程序，以继续研究 果蝇钾通道的分子特性。 以 由于果蝇基因组相对较小， 研究人员计划确定钾通道的整个补体 在这种生物体中，包括电压依赖性、钙依赖性和 内向整流型。 本提案的指导论点是， 与哺乳动物相比，果蝇似乎每种动物的成员都较少， 钾通道亚类 因此，我们可以研究具体的 五种已知类型的钾通道中的每一种在 果蝇系统，因为没有（已知的）并发症， 功能冗余 此外，一部小说的鉴定 果蝇钾通道亚类可能导致分离出 几种新的哺乳动物同源物。 长期计划是获得整个钾的'蓝图' 通道系统中的苍蝇，使任何一个渠道的贡献 亚型最终可以与其生理和行为 在成年生物体中的作用。 新的钾通道类型， 在蝇类中鉴定的基因也将随后在哺乳动物中鉴定。 每个 克隆的钾离子通道亚型的功能 其特征在于非洲爪蟾卵母细胞表达系统及其细胞 通过免疫学方法确定果蝇内的分布。 最后，利用显性-阴性突变的反向遗传方法， 将采用策略来确定每个渠道子类的角色， 苍蝇的发育和行为。

DESCRIPTION: This is a renewal application to continue studies on the molecular properties of potassium channels in Drosophila. By taking advantage of the relatively small genome size of the fruit fly, the investigator plans to define the entire complement of potassium channels in this organism, including voltage-dependent, calcium-dependent, and inward rectifier types. The guiding thesis in this proposal is that, in contrast to mammals, it seems that the fly has fewer members of each potassium channel subclass. One can therefore examine the specific contributions of each of the five known classes of potassium channels in the Drosophila system because there is no (known) complication due functional redundancy. In addition, the identification of a novel Drosophila potassium channel subclass may lead to the isolation of several novel mammalian homologues. The long-term plan is to obtain a 'blueprint' of the entire potassium channel system in the fly so that the contribution of any one channel subtype can eventually be related to its physiological and behavioral role in the adult organism. Novel potassium channel types that are first identified in flys will also be subsequently identified in mammals. Each of the cloned potassium channel subtypes will be functionally characterized in the Xenopus oocyte expression system and its cellular distribution within the fly will be determined by immunological methods. Finally, a reverse genetic approach using dominant-negative mutation strategy will be employed to identify roles of each channel subclass in fly development and behavior.