WOUND HEALING ACCELERATION USING NATURAL PRODUCTS

该更新提案描述了对具有cicatrizant（伤口愈合）活性的秘鲁植物进行跨学科，系统和有针对性的研究。 虽然愈合植物构成了 作为该提案的主要焦点，还将筛选提取物的潜在抗肿瘤活性。 秘鲁多样的地形、亚热带气候和独特的文化遗产 有助于使这个国家成为生物勘探的理想之地。 瘢痕形成剂可以帮助患有糖尿病、胃溃疡或静脉曲张溃疡等疾病的患者，这些患者的伤口不容易留下疤痕。 尽管有这些医疗福利， 对伤口愈合植物的科学研究可能有助于揭示受伤、愈合和治疗增生性疾病之间是否存在联系。 最近的研究发现了加强损伤和致癌之间相关性的证据。 研究结疤植物及其有效成分的作用机制， 揭示了能够促进组织再生的天然物质。 这项调查将阐明外部促进愈合机制引发的急性，也许慢性损伤。一个五步的方法来愈合伤口的天然产品已经 设计：1。安雷得草活性成分的表征及麻疯树胶乳的生物活性导向分级。 前者在上一次赠款期间被孤立 期 2.塔斯品碱和（+）-茴香醇的构效关系研究，这两种成分分别存在于巴豆和胡椒中。 后者是 在上一个赠款期间分离和鉴定。 3.细胞迁移和细胞增殖研究，以探索在Peperomia中发现的愈合原则的作用机制 galioides、Anredera diffusa和Jatropa curcas。 4.表征来自在先前试验期间未能显示体内伤口愈合活性的提取物的活性成分 但在我们的抗癌筛查试验中检测呈阳性。 这对应于来自Campsiandra angustifolia的潜在拓扑异构酶II抑制剂。 5.科学评价新鉴定的秘鲁植物具有愈合和抗癌活性的粗提物。 这项研究的长期目标是加强秘鲁和美国科学家之间的国际和多学科合作， 将保护生物多样性与对发现和开发改善人类健康的药物所需的药用植物进行可持续的经济管理联系起来。

This renewal proposal describes an interdisciplinary, systematic and targeted study of Peruvian plants with cicatrizant (wound-healing) activity. Although cicatrizant plants constitute the main focus of this proposal, extracts will also be screened for potential antineoplasic activity. The diverse topography, semitropical climate and unique cultural heritage of Peru contribute to making this country a desirable place for bioprospecting. Cicatrizant agents could help patients with illness such as diabetes, gastric ulcers or varicose ulcers, whose wounds do not scar easily. Notwithstanding these medical benefits, the scientific study of wound-healing plants may help uncover whether a connection can be established between injuries, healing and the treatment of proliferative disorders. Recent work has uncovered evidence that strengthens a correlation between injury and carcinogenesis. The study of cicatrizant plants and the mechanisms by which their active principle(s) operate, will reveal natural agents capable of promoting tissue regeneration. This investigation will shed light on externally promoted healing mechanisms triggered by acute and, perhaps chronic, injury. A five-step approach to wound-healing natural products has been designed: 1. Characterization of the active principle from Anredera diffusa and bioassay-guided fractionation of Jatropa curcas latex. The former was isolated during the previous grant period. 2. Structure-activity relationship studies on taspine and (+)-anymol, the two cicatrizant principles found in Croton lechleri and Peperomia galioides, respectively. The latter was isolated and identified during the previous grant period. 3. Cell migration and cell proliferation studies to probe mechanism of action for cicatrizant principles found in Peperomia galioides, Anredera diffusa and Jatropa curcas. 4. Characterization of the active principle from an extract who failed to show in vivo wound-healing activity during the previous grant period but tested positive in our anticancer screening assay. This corresponds to a potential topoisomerase II inhibitor from Campsiandra angustifolia. 5. Scientific evaluation of crude extracts from newly identified Peruvian plants with cicatrizant and anticancer activity. The long-term objective of this research is to strengthen the international and multidisciplinary collaboration between scientists from Peru and the United States, with the aim of linking conservation of biological diversity with sustained economic management of medicinal plants needed for the discovery and development of pharmaceuticals to improve human health.