MICROVASCULAR FLUID AND SOLUTE EXCHANGE

此RCDA应用程序的目的是为我的 作为一名独立调查员， 微循环 我的重点是研究 液体和溶质穿过毛细血管的经血管运动， 间隙 在许多病理条件下， 在流体和溶质在血管和血管之间的分配中， 血管外间隙 通过微血管系统的液体交换是 部分受间质流体静力学和细胞内分泌控制 压力 间质膨胀压力由 血管外血浆蛋白在间质凝胶中的分布 基质，由胶原蛋白和糖胺聚糖组成。 因此，我的目标是 研究血浆蛋白在间质中的转运和分布 凝胶基质，由胶原蛋白和糖胺聚糖组成。 因此，我的目标是 研究血浆蛋白的转运和分布， 在不同类型的水肿，并将数据与 介绍有关控制体内液体平衡的概念。 我们 初步研究表明，血浆蛋白通过 盐水膨胀后的间隙基质不同于 静脉压升高或毛细血管壁增厚后 血浆蛋白的渗透性。 我建议检验这样的假设： 血浆蛋白通过游离液体从血浆运送到淋巴 静脉充血或内皮细胞改变后的通道 渗透性，并在盐水膨胀后通过凝胶基质，或 慢性血浆置换 在麻醉的兔子中，我们将确定 示踪白蛋白和IgG从血浆至 淋巴管 我将研究兔子后爪的皮肤和骨骼肌。 我将比较血浆和血浆之间示踪白蛋白的平衡率， 淋巴液到血浆和间质间隙之间。 我还将测试 除了空间位阻外，还存在静电排斥的假设 从间质凝胶的部分中排除白蛋白。 这些结果 将提供有关组织间质基质作用的信息， 液体平衡以及提供深入了解水肿的病因 阵 这个项目也将成为我研究工作的重点 在接下来的5年里，作为一名专业的生理学家， 微循环领域的独立科学家

The intent of this RCDA application is to provide support for my development as an independent investigator in the area of microcirculation. My emphasis is directed towards the study of transvascular movement of fluid and solutes across capillaries and the interstitial space. In many pathological conditions, there is an imbalance in the partition of fluid and solutes between the vascular and extravascular spaces. Fluid exchange across the microvasculature is partially controlled by the interstitial hydrostatic and oncotic pressures. The interstitial oncotic pressure is determined by the distribution of extravascular plasma proteins in the interstitial gel matrix, composed of collagen and glycosaminoglycans. Thus, my goal is to study the transport and distribution of plasma proteins in the interstitial gel matrix, composed of collagen and glycosaminoglycans. Thus, my goal is to study the transport and distribution of plasma proteins in the interstitium during different types of edema and relate the data to the present concepts on the control of fluid balance in the body. Our preliminary studies indicate that the transport of plasma proteins through the interstitial matrix following saline expansion is different than that following increased venous pressure or increased capillary wall permeability to plasma proteins. I propose to test the hypothesis that plasma proteins are carried from plasma to lymph through free fluid channels following venous congestion or alterations in endothelial permeability, and through the gel matrix following saline expansion or chronic plasmaphoresis. In anesthetized rabbits we will determine the initial equilibration rates for tracer albumin and IgG from plasma to lymph. I will study both skin and skeletal muscle in the rabbit hindpaw. I will compare the equilibration rate for tracer albumin between plasma and lymph tothat between plasma and the interstitial space. I will also test the hypothesis that there is electrostatic exclusion, in addition to steric exclusion, of albumin from portions o the interstitial gel. These results will provide information on the role of the interstitial matrix in tissue fluid balance as well as provide insight into the etiology of edema formation. This project will also form a focus for my research efforts over the next 5 years as I develop my career as a professional physiologist and independent scientist in the field of microcirculation.