LINEAL ORIGIN OF NEURON GROUPS

候选人将从事专注于胚胎研究的职业生涯 克隆相关神经元的分化及其分化途径 在神经元形态、生理和生化(传递型)方面领先 与神经元中不同的解剖和生理联系有关 成熟的中枢神经系统的回路。拟议的实验将 解决我们已有的刻板印象神经元组的直系起源 最近在蝗虫血吸虫成虫神经节中的描述 Americana，以及不同表型性状的表达。 这些组中单独识别的神经元。一个主要的先决条件是 这些目标的实现是为了提高应聘者的熟练程度 在许多方法中，主要是与血统分析有关的方法 在胚胎中。这些方法的专业知识可以在埃默里大学学习到一部分 在大学，部分在同事的机构工作。一项研究 职业发展奖将极大地促进这一进程，通过提供 有更多研究活动机会的候选人(85%) 通过减少对教学和大学服务的承诺。我们的 具体目标包括对我们建议的一组神经元的研究 从单个神经母细胞胚胎发育而来。该组织约有20名成员 不同表型的神经元，包括谷氨酸能运动神经元， 节间中间神经元可能是GABA能的，是一种多支传出 这可能与其他昆虫的肽能神经元同源， 局部中间神经元的数量不确定。我们将描述解剖学上 和电生理特性的变化。 成人，这样我们就可以把每个人都识别为一个个体。我们将决定 哪些属性由组中的所有成员共享，哪些是 对某些人来说是独一无二的。然后我们将开始研究胚胎，以测试我们的 假设神经元是克隆相关的。为了追踪血统，我们将 使用常规技术在细胞内注射染料和 新开发的用改变的核苷BUDR标记技术。 用染料、BUDR和其他抗体进行标记将在 时间序列来确定各种不同来源的相对时间 神经元和获得不同的表型特征。

The candidate will pursue a research career that focuses on the embryonic differentiation of clonally-related neurons and on the way that differences in neuronal morphology, physiology and biochemistry (transmitter type) lead to differing anatomical and physiological connections in the neuronal circuitry of the mature central nervous system. Experiments proposed will address the lineal origins of stereotyped neuron groups which we have recently described in the adult ganglia of the grasshopper Schistocerca americana, and the expression of differing phenotypic traits in individually-identified neurons in these groups. A major prerequisite for the realization of these goals is for the candidate to develop proficiency in a number of methodologies, primarily those related to lineage analysis in the embryo. Expertise in these methods can be learned partially at Emory University and partially at the institutions of colleagues. A Research Career Development Award would greatly facilitate this process by providing the candidate with greater opportunity for research activities (85%) through reduction in commitments to teaching and university service. Our specific aims involve the study of one group of neurons that we suggest arise embryonically from a single neuroblast. The group contains some 20 neurons of diverse phenotype, including glutaminergic motor neurons, intersegmental interneurons that may be GABA-ergic, a multibranced efferent that may be homologous to peptidergic neurons from other insects and an indeterminate number of local interneurons. We will describe the anatomical and electrophysiological properties of the neurons in the group in the adult, so that we can identify each as an individual. We will determine which properties are shared by all members of the group and which are unique to some. We will then begin work on the embryo, to test our hypothesis that the neurons are clonally related. To trace lineage we will use conventional techniques for the intracellular injection of dye and newly-developed techniques for labeling with an altered nucleoside BUdR. The labeling with dyes, BUdR and other antibodies will be done in a closely timed series to determine the relative time of origin of the various neurons and of acquisition of differing phenotypic traits.