Quantitative three-dimensional imaging of membrane proteins

该项目旨在通过抗体成像质谱学这一新兴领域解决膜蛋白原位定量的挑战。该项目将开发定量三维成像的新方案，旨在消除由冻融和冷冻切片产生的组织学人工制品。膜蛋白参与了许多细胞功能，该项目希望通过对这些重要生物分子的研究提供新的见解，增加我们对这些基本生物过程的了解。在三维空间中跟踪蛋白质的异质性将为我们理解系统生物学提供显著的益处，并将使包括制药工业在内的许多领域受益。

This project aims to address the challenge of in-situ quantification of membrane proteins through the emerging field of antibody-imaging mass spectrometry. The project will develop new protocols for quantitative three-dimensional imaging that aim to negate histological artifacts created by freeze-thaw and cryo-sectioning. Membrane proteins are involved in numerous cellular functions and this project expects to increase our knowledge of these fundamental biological processes by providing new insights into the study of these essential biomolecules. Tracking protein heterogeneity in three-dimensions will provide significant benefits to our understanding of systems biology and will benefit numerous area, including the pharmaceutical industry.