Characterisation of vital carbohydrate synthases in pathogenic oomycetes

本项目旨在了解鱼类病原菌--寄生腐霉控制细胞壁稳定性的机制。涉及细胞壁生物合成的重要酶的生化特性和功能将在生物化学、微生物学、细胞生物学和结构生物学的界面上使用创新的方法来确定。下一代离子迁移质谱仪将被用来解决传统技术无法解决的具有挑战性的结构问题。预期成果包括具有挑战性的膜蛋白方面的新知识，从而能够开发水产养殖疾病控制的新策略。这些数据也可能适用于保护作物免受相关植物病原体的侵害。

This project aims to understand the mechanisms that control cell wall stability in the fish pathogen, Saprolegnia parasitica. The biochemical properties and function of vital enzymes involved in cell wall biosynthesis will be determined using innovative approaches at the interface of biochemistry, microbiology, cell biology, and structural biology. Next generation ion mobility mass spectrometry will be used to solve challenging structural questions that cannot be tackled with conventional techniques. Expected outcomes include new knowledge on challenging membrane proteins that allows development of novel strategies for disease control in aquaculture. The data may also be applicable to crop protection from related plant pathogens.