THE REGULATION OF V ALPHA 7 EXPRESSING T CELLS

我们观察到外周T细胞表达V 在C57BL/10小鼠中，alpha7基因的表达严重不足。这 考虑到Vα7基因占Vα的8%-10%，这是令人惊讶的 生殖系基因库。这项拨款中提议的实验 应用程序试图确定以下机制或过程 调节小鼠体内表达Vα7的T细胞的数量 外围设备。 设计了一组os实验，以确定V字母7 基因带有阻止其重排的结构缺陷 或者高效的转录。 第二组实验被设计来估计频率 胸腺细胞和外周T细胞中Vα7表达细胞的比较 细胞；这些实验关注的是耐受的潜在作用 和/或胸腺选择决定成熟细胞的数量， 外周Vα7表达的T细胞。

We have made the observation that peripheral T cells expressing V alpha7 genes are highly underrepresented in C57BL/10 mice. This is surprising considering V alpha7 genes comprise 8%-10% of V alpha germline gene pool. The experiments proposed in this grant application seek to determine the mechanism or process which regulates the number of V alpha7-expressing T cells in the periphery. A set os experiments is designed to determine whether the V alpha7 genes carry a structural defect that prevents their rearrangement or efficient transcription. A second set of experiments is designed to estimate the frequence of V alpha7-expressing cells among thymocytes and peripheral T cells; these experiments focus on the potential role of tolerance and/or thymic selection in determining the number of mature, peripheral V alpha7-expressing T cells.