ETHANOL--SUPPRESSION OF MYCOBACTERIAL RESISTANCE

大量饮酒会增加患呼吸道疾病的风险 疾病和结核病，可能与增加的 与人类免疫缺陷相关的分枝杆菌病的发病率 病毒感染。多达50%的死于艾滋病的人 已经感染了禽类分枝杆菌。这项工作是一种延伸 我实验室的研究表明，微分表达式 小鼠巨噬细胞和人MHC-II类糖蛋白的表达 单核细胞可能与先天激活状态的差异有关。 巨噬细胞的差异和对分枝杆菌感染的抵抗力的差异。 为了实现我们的目标，我们将利用 分枝杆菌病，利用小鼠的同源品系 分枝杆菌的对分枝杆菌在体内的生长具有抵抗力或易感性的研究 已经表明，酒精的消耗或巨噬细胞的治疗在 体外乙醇对分枝杆菌的抑制作用 抵抗。要了解乙醇对增加的 对分枝杆菌生长的敏感性我们将：确定影响 酒精摄入对脾内禽分枝杆菌生长的影响 和感染小鼠的肺组织；测定酒精摄入对小鼠肺组织的影响 巨噬细胞介导的抗分枝杆菌机制及测定 乙醇体外处理对人参皂苷抗分枝杆菌活性的影响 脾和肺巨噬细胞及其对细胞因子的反应。

Heavy use of alcohol is associated with an increase risk of respiratory disease and of tuberculosis and may be associated with the increased incidence of Mycobacterial disease associated with human immunodeficiency virus infection. As many as 50% of the individuals who have died of AIDS have been infected with Mycobacterium avium. This work is an extension of work in my laboratory that has shown that the differential expression of MHC class II glycoproteins by both murine macrophages and human monocytes may be related to differences in the innate state of activation of macrophages and differences in resistance to Mycobacteria] infection. To accomplish our goals we will take advantage of a model for Mycobacterial disease that utilizes congenic strains of mice that are resistant or susceptible to the in vivo growth of Mycobacteria. Studies have shown that consumption of ethanol or treatment of macrophages in vitro with ethanol results in the suppression of anti-mycobacterial resistance. To understand the role of ethanol on the increased susceptibility to Mycobacterial growth we will: Determine the effect of ethanol consumption on the growth of Mycobacterium avium in the spleen and lung of infected mice; determine the effect of ethanol consumption on macrophage mediated anti-mycobacterial mechanisms and determine the effect of in vitro ethanol treatment on the antimycobacterial activity of splenic and lung macrophages including their response to cytokines.