PRODUCTION AND ANALYSIS OF HISTOCOMPATIBILITY MUTANTS

这个项目旨在维持、发展和扩大一个老鼠群体 其携带影响皮肤移植物移植的基因突变。 一 大量线路已经到位、完成或正在建设中， 发展 这些包括携带H-2突变的菌株以及 非H-2基因突变 新的突变将通过筛选 ENU处理或未处理的品系C57BL/6Kh雄性小鼠的后代， BALB/cKh. 此外，还有许多其他的近交、同源和重组的 菌株将作为系统的一部分不断筛选，以监测其 遗传完整性和突变体也可以从它们中恢复。 突变体将进入免疫遗传学分析，两者都是正式的 合作努力，并将其提供给其他调查人员 应要求 正式合作者包括斯坦利·纳森博士 （蛋白质突变基因产物的生物化学和结构分析） 和DNA水平），Ian McKenzie博士（突变基因的血清学分析 Kees Melief博士（突变的功能后果， 细胞介导的测定，如CML，MLR和MHC限制），Peter博士 Wettstein（寻找由于病毒感染而导致的整合病毒序列的变化） 涉及非H-2基因突变的突变）和Byung Kim博士 （协助电泳技术）。 我们将专门研究最近发现的H-2dm6突变 （它已经失去了Dd，但保留了Ld，显然也保留了Rd），看看D是否 除了D、L和R以外的区域产品可以识别，这些产品在 变种人 我们还建议尝试提高一些同种抗血清 作为未来分析的一步， 这些基因。

This project seeks to maintain, to develop and to expand a colony of mice which carry mutations of genes which affect skin graft transplantation. A substantial number of lines are already on hand, completed or under development. These include strains bearing H-2 mutations as well as non-H-2 gene mutations. New mutations will be sought by screening the progeny of ENU-treated or untreated male mice of strains C57BL/6Kh and BALB/cKh. In addition a number of other inbred, congenic and recombinant strains will be continually screened as part of a system to monitor their genetic integrity and mutants may be recovered from them as well. Mutants will be entered into immunogenetic analyses, both as part of formal collaborative efforts and by making them available to other investigators upon request. The formal collaborations include Dr. Stanley Nathenson (biochemical and structural analysis of mutant gene products at the protein and DNA levels), Dr. Ian McKenzie (serological analyses of mutant gene products), Dr. Kees Melief (functional consequences of the mutations for cell-mediated assays such as CML, MLR and MHC restriction), Dr. Peter Wettstein (looking for changes in integrated viral sequences as a result of mutations involving mutations of non-H-2 genes), and Dr. Byung Kim (assisting in electrophoretic techniques). We will be specifically examining the recently reovered H-2dm6 mutation (which has lost Dd, but retained Ld and apparently Rd as well) to see if D region products other than D,L and R can be identified which are missing in the mutant. We also propose attempting to raise alloantisera against some of the minor histocompatibility mutants as a step toward future analyses of these genes.