MOLECULAR ANALYSIS OF HEPATITIS B VIRUS

提出的研究可以分为两个主要方面。第一 关注对乙肝病毒(乙肝病毒)生命周期的研究。的目的是 这项工作是对病毒以及它是如何 在肝细胞中自我复制的功能。它包括(1)一个 乙肝病毒基因的综合分析(表面抗原(表面抗原)、核心(HBcAg)) 抗原、DNA聚合酶？和一个750B RNA)及其在体内和 在试管中。此外，我们还将确定“e”抗原的性质。 (2)病毒假定复制形式的分析及控制 病毒的复制。(3)22 nm的分子表征 乙肝表面抗原颗粒。(4)亲肝的分子基础研究--a 寻找一个可能的乙肝病毒受体。(5)体外培养体系的建立 用于研究病毒的传染性和营养生长。第二 主要方面涉及对乙肝病毒在疾病中的作用的分析。其目的是 这些研究的重点是阐明乙肝病毒诱导的分子基础。 并直接检测乙肝病毒是否为PHC的病原体。 该建议包括(1)各种病毒DNA形式的研究和 感染者肝脏和血清中的病毒来源的乙肝表面抗原颗粒 患者--各种形式的肝炎(自限性肝炎、暴发性肝炎 肝炎、慢性肝炎、携带者)和原发性肝细胞 癌症(HPC)。(2)乙肝病毒序列的结构和表达分析 整合到肝细胞瘤基因组DNA中的基因及其生化后果 这种整合。这可能揭示出一个基本的 本体论过程。(3)测试单字或双字的表达 病毒基因会导致细胞损伤。这些研究将有助于 据我们对病毒和病毒性疾病的了解。它们可能会照亮 世界上最常见的癌症的病因学。此外，他们还将 有助于了解基因结构的基本分子细节，以及 哺乳动物细胞中基因表达的调控及其分子机制 宿主组织特异性。

The proposed research can be divided into two main aspects. The first concerns studies on the hepatitis B virus (HBV) life cycle. The aim of this work is a comprehensive understanding of the virus and how it functions to replicate itself in the hepatic cell. It includes (1) a comprehensive analysis of HBV genes (surface (HBsAg), core (HBcAg) antigens, DNA polymerase?, and a 750b RNA) and their expression in vivo and in vitro. In addition, we will determine the nature of the "e" antigen. (2) Analysis of presumed replicative forms of the virus and the cotnrol of replication of the virus. (3) The molecular characterization of the 22nm HBsAg particle. (4) A study of the molecular basis of hepatotropism--a search for a putative HBV receptor. (5 Development of an in vitro system for studying infectivity and vegetative growth of the virus. The second major aspect concerns an analysis of the role of HBV in disease. The aim of these studies is to elucidate the molecular basis of HBV induced hepatitis and to directly test whether HBV os tje causative agent of PHC. The proposal includes (1) studies of the various viral DNA forms andof virus-derived HBsAg particles in the liver and serum of infected patients--various forms of hepatitis (self limited hepatitis, fulminant hepatitis, chronic hepatitis, carriers), and in primary hapatocellular carcinoma (HPC). (2) Analysis of structure and expression of HBV sequences integrated in genomic DNA of hapatomas and the biochemical consequences of this integration. This may reveal the molevular outline of a basic ontological process. (3) Tests whether the expression of single or pairs of virus genes will cause cellular lesions. These studies will contribute to our knowledge of viruses and viral diseases. They may illuminate the etiology of the world's most prevalent cancer. In addition they will contribute undersanding of basic molecular details of gene structure, and regulation of gene expression in mammalian cells as well as the molecular specifics of host-tissue specificity.