CELLULAR CONTROL OF EB VIRUS IN RHEUMATOID ARTHRITIS

类风湿关节炎(RA)患者的淋巴细胞受损 他们控制EB病毒(EBV)感染的能力。EBV诱导 体外感染后的淋巴母细胞转化更快 在RA细胞中，自发淋巴母细胞转化发生得更多 从类风湿性关节炎患者的血液中获取的细胞中 来自健康个体的细胞。EB病毒诱导的淋巴母细胞的调控 核扩散可分为“早期”和“晚期”阶段，这两个阶段似乎 由细胞事件和由衍生于 激活的细胞。我们计划继续剖析这些监管机构 事件，比较来自健康个体的细胞功能与 来自类风湿性关节炎患者。对直接细胞介导的事件的研究将是 针对负责任的监管机构的表型特征 亚群和确定RA细胞中的调节缺陷是否 EBV的特异性或更普遍的控制B病毒的能力缺陷 细胞增殖。对可溶性调节因子的研究将包括 分析其产生所需的相互作用的细胞群体； 前列腺素在类风湿关节炎能力减弱中的作用分析 细胞产生它；以及分析作为干扰素的可溶性因子。 类风湿关节炎EB病毒感染调控异常的研究 应该继续提供关于免疫调节的新信息，因为 慢性自身免疫性疾病。因为它的无处不在、持久性和 改变免疫反应的能力，EBV是一种吸引人的有机体 考虑一下自身免疫性疾病。而EBV感染不太可能是 RA中的启动事件，它可能凭借其调节能力 免疫反应和增强类风湿因子的产生，在 这种疾病的长期存在。

Lymphoid cells from patients with rheumatoid arthritis (RA) are impaired in their ability to control Epstein-Barr virus (EBV) infection. EBV-induced lymphoblast transformation following in vitro infection occurs more rapidly in RA cells, and spontaneous lymphoblast transformation occurs more frequently in cells obtained from the blood of RA patients than it does in cells from healthy individuals. The regulation of EBV-induced lymphoblast proliferation can be divided into "early" and "late" phases which appear to be mediated by cellular events and by soluble factors derived from activated cells. We plan to continue the dissection of these regulatory events, comparing the function of cells from healthy individuals with those from RA patients. Studies of the direct cell-mediated events will be directed at phenotypically characterizing the responsible regulatory subpopulations and determining if the regulatory defects in the RA cells is specific for EBV or a more generalized defect in the ability to control B cell proliferation. Studies of the soluble regulatory factor will include analysis of the interacting cell populations required for its generation; analysis of the role of prostaglandins in the diminished capacity of RA cells to generate it; and analysis of the soluble factor as an interferon. Studies of the abnormal regulation of EBV infection in rheumatoid arthritis should continue to yield new information regarding immunoregulation in that chronic autoimmune disorder. Because of its ubiquity, persistence, and ability to alter immune responses, EBV is an attractive organism to consider in autoimmune disease. While EBV infection is not likely to be the initiating event in RA, it may, by virtue of its ability to modulate immune responses and enhance rheumatoid factor production, play a role in the perpetuation of the disease.