ACTIVE SUGAR TRANSPORT IN RENAL CELLS

胰岛素对肾脏糖转运和代谢的调节作用 将研究：1.糖代谢关键酶的分布 (e.g.,己糖激酶、葡萄糖-6-磷酸酶和果糖-1，6-二磷酸酶， 磷酸烯醇丙酮酸羧激酶和丙酮酸脱氢酶将被 在正常、低胰岛素血症的肾皮质中测定 （链脲霉素处理的）和高胰岛素血症（肥胖，Zucker）大鼠。 数据 将与胰岛素对糖的影响的研究结果相关联 在肾皮质小管的反腔细胞面的运输，使用 选择性进入不可代谢的3-O-甲基-D-葡萄糖作为标记。 2. 积极的糖转运（和新生），以及胰岛素和 参与调节肾脏糖转运和代谢的其他药物 将在生长于大肠杆菌中的肾上皮细胞的LLC-PK 1系中进行研究。 体外 3.果糖主动转运的机制将通过使用 新生仔猪和羔羊肾皮质微绒毛囊泡; 果糖是这些哺乳动物物种的新生儿中的主要血浆糖， 它的水平不受胰岛素的影响。 4.小说现象的机制 糖分泌在肾脏（和肠）的比目鱼 （Pseudopleuronectes americanus）将被阐明。

The insulin effect on the regulation of renal sugar transport and metabolism will be studied: 1. The distribution of key enzymes of carbohydrate metabolism (e.g., hexokinases, glucose-6-phosphatase, and fructose-l,6-diphosphatase, phospho-enol-pyruvate carboxykinase and pyruvate dehydrogenase will be determined in the renal cortex of normal, hypoinsulinemic (streptozotocin-treated) and hyperinsulinemic (obese, Zucker) rats. The data will be correlated with results of studies on the insulin effect on sugar transport at the antiluminal cell face of renal cortical tubules, using the selective entry of the non-metabolizable 3-O-methyl-D-glucose as a marker. 2. Active sugar transport (and gluconeogenesis), and the effect of insulin and other agents involved in the regulation of renal sugar transport and metabolism will be investigated in the LLC-PKl line of renal epithelial cells grown in vitro. 3. The mechanism of active fructose transport will be determined using vesicles of microvilli from the renal cortex of newborn piglets and lambs; fructose is the dominant plasma sugar in newborns of these mammal species, and its level is not affected by insulin. 4. The mechanism of the novel phenomenon of sugar secretion in the kidney (and intestine) of the flounder (Pseudopleuronectes americanus) will be elucidated.