Expandable Clinical Grade Feeder Cells for hESc Derivation

我们的目标是产生无限供应的永生化临床级人真皮成纤维细胞（hDF）用于衍生cGMP hESc系。人胚胎干细胞（hESc）具有用于临床治疗的巨大潜力，但目前的hESc系尚未在适合临床使用的条件下产生。我们将利用我们成功的TSB资助的可行性研究来产生合适的永生化人真皮成纤维细胞（hDF），用于衍生cGMP hESc系。我们将永生化2个原代（临床级）hDF细胞系，这些细胞系已被证明可在独特的培养基系统中支持cGMP hESc细胞系的培养和衍生，使用我们在研究水平上显示的生成hESc支持性饲养细胞的方法。将对永生化细胞进行新cGMP hESc系的衍生试验，并将提供可销售的cGMP饲养细胞供应，用于产生治疗用hESc。

We aim to generate an inexhaustible supply of immortalised clinical grade human dermal fibroblasts (hDFs) for derivation of cGMP hESc lines. Human Embryonic stem cells (hESc) have great potential for use in clinical therapies but current hESc lines have not been generated under conditions suitable for clinical use. We will exploit our successful TSB-funded feasibility study to generate suitable immortalised human dermal fibroblasts (hDFs) for derivation of cGMP hESc lines. We will immortalise 2 primary (clinical grade) hDF lines, already shown to support culture and derivation of cGMP hESc lines in a unique media system, using a method shown by us to generate hESc-supportive feeder cells at the research level. The immortalised cells will be tested for derivation of new cGMP hESc lines and will provide a supply of marketable cGMP feeder cells for use in generating hESc for therapy.