Frequency-Modulated Stimulated Raman Scattering Microscopy for Label-Free Functional Imaging In-Planta

多年来，许多学科的科学家一直梦想着能够在生物组织深处创建活细胞的图像，并识别其中的化学物种。这个梦想正在成为现实--物理学家正在开发尖端的光学显微镜技术，使生物医学科学家能够观察活组织内部，并根据生物分子与光相互作用的方式的细微差异来识别生物分子。不幸的是，植物科学界一直未能从这些技术中受益。植物已经进化成高效的光吸收者，并含有产生光信号的色素，这种光信号压倒了光和感兴趣的生物分子之间的微妙相互作用。我们建议开发一种新的检测方案，该方案将克服在存在强烈光吸收的情况下成像的根本限制，并使学术和工业研究人员都能从这项尖端技术中受益。

The ability to create images of living cells deep within biological tissues and to identify chemical species within them has been a dream for scientists in many disciplines for many years. This dream is becoming reality - physicists are developing cutting-edge optical microscopy techniques that allow biomedical scientists to look inside living tissues and identify biomolecules based on subtle differences in the way they interact with light. Unfortunately, the plant science community has not been able to benefit from these techniques. Plants have evolved to be highly efficient absorbers of light, and contain pigments that generate an optical signal that overwhelms the subtle interactions between the light and the biomolecules of interest. We proposed to develop a new detection scheme that will overcome the fundamental limitations of imaging in the presence of strong optical absorption and allow both academic and industrial researchers to benefit from this cutting-edge technology.