IMMUNOLOGIC STUDIES RELATED TO MALIGNANCY

我们已经开发了一个系统来简化人类细胞表面的研究 结构的程序，允许研究抗原由于个人 染色体 一种人与中国人的性状，体细胞杂种，其中含有 11号染色体是人类唯一的染色体。 这个混血儿 表达人类细胞表面抗原的细胞， 系统来研究这些成分， 生物化学和遗传学的方法。 我们开发了一个删除克隆 11号染色体的面板，这使我们能够进行各种各样的 区域绘图研究，包括开发一种新方法， 低重复人类DNA序列 单克隆抗体的生产 使我们能够进一步表征这些抗原， 由11号染色体编码的抗原。 我们已经开始了实验， 使我们能够克隆其中两种抗原的基因。 我们最近进行了类似的研究，使用的是只保留 人类3号和21号染色体 正在为这些项目开发删除小组， 染色体，并已用于区域绘图。 这类研究 应该能够快速和明确地鉴定大量品种 人类细胞表面抗原，可能会影响恶性行为， 人体细胞 这些基因的染色体定位和生化特性 遗传决定子可能有助于阐明抗原的遗传关系 参与恶性反应。 （简体中文）

We have developed a system to simplify the study of human cell surface structures by procedures which permit study of antigens due to individual chromosomes. A human-Chinese hameter, somatic cell hybrid, which contains chromosome 11 as its only human chromosome has been produced. This hybrid, which expresses human cell surface antigens, has been employed as a model system to study these components using a combined immunological, biochemical, and genetic approach. We have developed a deletion clone panel of chromosome 11, which has enabled us to conduct a variety of regional mapping studies including development of a new method using a low-repeat human DNA sequence. The production of monoclonal antibodies has allowed us to further characterize these antigens and to identify new antigens encoded by chromosome 11. We have begun experiments which should enable us to clone the genes for two of these antigens. We have recently carried out similar studies using hybrids that retain only human chromosomes 3 and 21. Deletion panels are being developed for these chromosomes and have been used for regional mapping. Studies of this kind should make possible rapid and definitive identification of a large variety of human cell surface antigens which may influence malignant behavior of human cells. The chromosomal location and biochemical identity of such genetic determinants may help elucidate genetic relationships of antigens involved in malignant response. (CS)