RECEPTORS FOR IMMUNOGLOBULIN G ON LEUKOCYTES

这项建议的目的是描述Fc的受体。 自然自卫中人细胞效应物上的免疫球蛋白G片段 机械装置。Fc受体的两大类：单体的Mo-FCR 免疫球蛋白，存在于单核细胞和经干扰素处理的髓系细胞上， 成熟髓系细胞和NK/K细胞上存在的复合型免疫球蛋白的PMN-FCR， 将以生物化学为特征。它们的分子结构、酶 外周血的敏感性、细胞内外分布 细胞亚群、正常骨髓、白血病细胞和粒单核细胞 将定义行。FCR、其基因表达的调控 在不同的细胞类型中的功能，以及内部可能的异质性 我们将对它们进行分析。实验方法包括生产 针对不同抗原表位的额外单抗 PMN-FCR和抗Mo-FCR。抗体将被用来沉淀 Fc结合蛋白，研究其Ig结合特性，以确定 它们在不同细胞类型上的差异表达，以检测 携带FCR的淋巴细胞亚群，并确定 表达受体的骨髓单核细胞分化。这个 不同的FCR类型在FCR依赖功能中的作用如下 调查过了。编码不同基因组的基因(S)的染色体定位 将尝试使用单抗进行FCR以确定 人-鼠体细胞杂交的表型和筛选克隆。(CS)

The objective of this proposal is to characterize the receptors for the Fc fragment of IgG present on human cell effectors in natural self-defense mechanisms. The two major classes of Fc receptors, Mo-FcR for monomeric immunoglobulins, present on monocytes and interferon-treated myeloid cells, and PMN-FcR for complexed IgG present on mature myeloid and NK/K cells, will be characterized biochemically. Their molecular structure, enzyme sensitivity, intra-\and extra-cellular distribution on peripheral blood cell subsets, normal bone marrow, leukemic cells, and myelomonocytic cell lines will be defined. The control of the expression of FcR, their function in the different cell types, and the possible heterogeneity within them will be analyzed. The experimental approach includes the production of additional monoclonal antibodies directed against distinct epitopes of PMN-FcR and against Mo-FcR. The antibodies will be used to precipitate the Fc-binding proteins, to study their Ig-binding characteristics, to define their differential expression on the different cell types, to detect lymphocyte subpopulations bearing FcR, and to define the stage of myelomonocytic differentiation at which the receptors are expressed. The role of the different FcR types in FcR-dependent functions will be investigated. Chromosomal mapping of the gene(s) encoding the different FcR will be attempted using the monoclonal antibodies to determine the phenotype and select clones of human-mouse somatic cell hybrids. (CS)