MECHANISM OF MACROPHAGE RECOGNITION OF TUMOR CELLS

巨噬细胞被认为是肿瘤监测的重要组成部分。 在活体内。它们被认为是肿瘤的一种极好的机制 破坏，因为它们会杀死肿瘤细胞，而离开正常细胞 安然无恙。巨噬细胞裂解肿瘤细胞的过程现在被认为 至少是两个可识别的步骤--承认和约束步骤 随后巨噬细胞释放细胞毒介质。虽然 巨噬细胞细胞毒性的步骤已经被很好地识别， 巨噬细胞与肿瘤结合的分子或识别结构 细胞或如何区分正常细胞和肿瘤细胞尚不清楚。 研究已经分离出了SV40转化的肿瘤细胞，这些细胞对 抵抗脂多糖激活的小鼠巨噬细胞的体外细胞溶解。这个 特异性RNA肿瘤病毒诱导的gp70的存在与 抵抗巨噬细胞的裂解。使用Friend白血病病毒 (Flv)转化的细胞具有不同的gp70，与易感细胞相关 对巨噬细胞有细胞毒作用，与巨噬细胞无抵抗力 发现了FLV转化的细胞。 研究表明，gp70可能参与肿瘤识别，但如何 它是否牵涉其中尚不确定。此外，其他分子的参与 并没有被完全反驳。在这项研究中，计划是 探讨巨噬细胞识别肿瘤细胞的机制(S) 用生化方法确定被杀死或未被杀死的细胞之间的差异 激活的巨噬细胞。(MB)

Macrophages are believed to be an important component of tumor surveillance in vivo. They are thought to be an excellent mechanism of tumor destruction, as they will kill tumor cells while leaving normal cells undisturbed. The process of macrophage lysis of tumor cells is now thought to be at least two identifiable steps--a recognition and binding step followed by the release of cytotoxic mediators by the macrophage. Although the steps in macrophage cytotoxicity have been well identified, the molecules or recognition structures that macrophages use to bind to tumor cells or to discriminate normal cells from neoplastic cells are unknown. Studies have isolated SV40-transformed tumor cells that were susceptible or resistant to in vitro cytolysis by LPS-activated murine macrophages. The presence of specific RNA tumor virus-induced gp70 was correlated with the resistance to lysis by macrophages. Using Friend leukemia virus (FLV)-transformed cells a different gp70, associated with cells susceptible to macrophage cytotoxicity and not with macrophage resistant FLV-transformed cells, was found. The studies suggest that gp70 may be involved in tumor recognition, but how it is involved is uncertain. Furthermore, involvement of other molecules has not been absolutely disproven. During this research, plans are to investigate the mechanism(s) of macrophage recognition of tumor cells and to biochemically define differences between cells killed or not killed by activated macrophages. (MB)