NOVEL HUMAN ENDOGENOUS RETROVIRUSES

本补助金申请中提出的研究 继续和扩展我们正在进行的隔离工作， 新的内源性基因家族的特征和表达 逆转录病毒 为此，我们计划分离和表征一种 据称是一类新的人类内源性逆转录病毒， 已经用禽逆转录病毒特异性基因探针鉴定出， 我们以前用来识别新的鸟类种类， 内源性逆转录病毒 我们的研究内容包括：（1） 禽逆转录病毒相关的人内源性病毒的分离 通过分子克隆和核苷酸鉴定的家族 序列分析;（2）表征的程度和性质， 这种额外的人类内源性基因家族的表达 正常和恶性人类细胞类型中的逆转录病毒;和（3）a 比较这种新的内源性逆转录病毒家族以及 其他先前鉴定的人类内源性 逆转录病毒与“C”型病毒粒子产生的各种 人类肿瘤，包括睾丸生殖细胞肿瘤， 将胚胎癌细胞群分化为 确定这些细菌产生的“C”型颗粒是否 细胞肿瘤显示出与任何家族的任何序列同源性 人类内源性病毒。 我们计划采用的方法 为实现这些目标，在很大程度上， 在我们的实验室中实现，包括各种 理化、酶学和杂交技术 可用于分析DNA、RNA和蛋白质，包括 核苷酸序列分析

The studies proposed in this grant application exemplify a continuation and extension of our ongoing work on the isolation, characterization and expression of new families of endogenous retroviruses. To this end, we plan to isolate and characterize a purportedly novel class of human endogenous retroviruses that we have identified with avian retrovirus-specific genetic probes that we have previously used to identify new classes of avian endogenous retroviruses. Our proposed studies include: (1) isolation of the avian retrovirus-related human endogenous virus family by molecular cloning and characterization by nucleotide sequence analysis; (2) characterization of the extent and nature of expression of this additional family of human endogenous retroviruses in normal and malignant human cell types; and (3) a comparison of this novel endogenous retrovirus family as well as the other previously identified families of human endogenous retroviruses with "C" type virion particles produced by various human tumors including testicular germ cell tumors and differentiating populations of embryonal carcinoma cells to determine whether the "C" type particles produced by these germ cell tumors exhibit any sequence homology to any of the families of human endogenous viruses. The methodologies that we plan to employ to achieve these objectives are, for the most part, already implemented in our laboratory and include a variety of physiochemical, enzymological and hybridization techniques available for the analysis of DNA, RNA, and protein, including nucleotide sequence analysis.