BREAST CANCER BRAIN METASTASIS

脑部肿瘤转移是一种比较常见的情况。 然而，在一些常见的系统性癌症中，预后 尽管最近进行了治疗，但患者的病情仍然严峻 预付款。这个项目建议检查转移的大脑。 来自人和大鼠乳腺癌的肿瘤，在 它们的生物学、病理学和生化方面的条件 属性。最初的重点是开发一种相关的动物 利用13762NF乳房建立大鼠脑转移瘤模型 腺癌。初步研究表明，大脑 通过重复选择定植肿瘤细胞变异体 将肿瘤细胞接种到颈动脉的连续循环 动脉，采集脑瘤结节和体外生长 组织培养。主要的重点将是比较大鼠的大脑 转移性肿瘤细胞对人乳腺癌脑的影响 病变及相应的原发肿瘤和其他 其他器官的远处转移。各种可能的路线 肿瘤细胞在脑内的转移性扩散也会 调查过了。各种转移的脑瘤细胞将是 检查它们的生物学特性、病理学和 卡里奥型。此外，肿瘤的细胞表面成分 将对细胞进行检查，以确定是否表达 糖蛋白、蛋白质、蛋白多糖或糖脂相关 它们的大脑定植能力。几个细胞表面 成分已经被证明是由这两个 人和大鼠乳腺癌细胞及其改变 表达水平可反映肿瘤细胞的转移能力。 这些结果的推广可能会使我们对 转移性脑肿瘤的种植、生长和定植 以及治疗中的新方法。

Tumor metastasis to the brain is a relatively frequent occurance in a number of common systemic cancers, however, the prognosis of afflicted patients remains grim despite recent therapeutic advances. This project proposes to examine metastatic brain tumors from mammary carcinoma of human and rat origin, in terms of their biological, pathological, and biochemical properties. An initial focus is to develop a relevant animal metastatic brain tumor model in rat using the 13762NF mammary adenocarcinoma. Preliminary studies have suggested that brain colonizing tumor cell variants are being selected by repeated sequential cycles of inoculating tumor cells into the carotid artery, harvesting the brain tumor nodules and in vitro growth in tissue culture. A major emphasis will be to compare the rat brain metastatic tumor cells to human mammary carcinoma brain lesions and also to the appropriate primary tumor and other distant metastases in other organs. The various possible routes of metastatic spread of the tumor cells in the brain will also be investigated. The various metastatic brain tumor cells will be examined for their biological properties, pathologies, and kariotypes. Furthermore, the cell surface components of tumor cells will be examined to determine if the expression of glycoproteins, proteins, proteoglycans, or glycolipids correlates with their brain colonizing ability. Several cell surface components have been demonstrated to be expressed by both human and rat breast carcinoma cells, and their altered expression may reflect the tumor cells metastatic capabilities. Extension of these results may lead us to new understanding of metastatic brain tumor implantation, growth, and colonization and to new approaches in therapy.