BBSRC Embrapa - Understanding the importance of the wheat root microbiome for sustainable crop production.

英国团队将由3名驻Rothamsted的科学家组成：Tim Mauchline博士(英国首席调查员)、Penny Hirsch教授和Ian Clark先生。来自EmbRapa环境的巴西团队由三名科学家组成：Rodrigo Mendes博士(巴西首席研究员)、Vanessa Nessner博士和Maike Rossmann博士。整个团队拥有执行根际元基因组学和小麦微观世界实验所需的互补和重叠的技能集。毛奇林是一名微生物学家，在根际微生物学和包括小麦在内的各种作物的微观世界方面拥有专业知识。赫希教授是一位土壤微生物学家，在土壤养分循环方面拥有丰富的经验。伊恩·克拉克先生是一位具有生物信息学技能的微生物学家。巴西队。门德斯是一位具有农业系统元基因组学技能的分子微生物学家。内斯纳博士是一位拥有生物信息学技能的分子生物学家。Maike Rossman博士是一位具有小麦栽培经验的土壤微生物学家。这项泵启动提案旨在继续2014年5月Tim Mauchline博士访问EmbRapa Environment的Rodrigo Mendes博士的实验室三周后建立的合作关系，资金来自ISIS。事实证明，毛奇林博士的访问非常有用，因为它使他得以融入EmbRapa集团。Mauchline博士和Mendes博士以及该小组的其他成员进行了多次讨论，以确定未来根际微生物组研究与小麦产量有关的方向。访问的直接结果是，EmbRapa Environment的博士后科学家Vanessa Nessner博士加入了Rothamsted团队，为期五个月(2015年12月至5月)，研究健康和患病植物中的小麦微生物组，她的工作将为泵启动研究提供平台。此外，曾在Rothamsted团队担任一年博士后职位的Maike Rossmann博士现在在EmbRapa环境的Mendes博士的实验室工作，致力于不同土壤类型小麦根际的元基因组学研究。因此，我们在Rothamsted和EmbRapa环境微生物学实验室之间开发了一个功能齐全的团队单位，具有互补的技能，本提案希望在此基础上进行。主要的现场实验已经在Rothamsted建立，不需要额外的成本，因为已经允许对其进行采样。研究费用用于土壤DNA提取试剂盒、扩增子测序和比较盆栽实验。有关更多信息，请参阅JOR。小麦采样、土壤DNA提取和盆栽研究分析将是一项合作工作，因为来访的巴西科学家EmbRapa和Rothamsted团队将在他们访问英国的一个月期间在Rothamsted田间试验和实验室中共同进行这些工作。申请的主要费用将是合作伙伴在巴西和英国之间的旅行。Mendes博士、Rossmann博士和Nessner博士将前往英国讨论和撰写完整的拨款提案，此外还将进行上述合作领域和实验室工作。我们还将邀请另外三位科学家在一个小型研讨会上提供建议(来自英国的Phil Poole教授和Gary Bending博士，以及来自巴西的Fernando Andreote博士)。提议Rothamsted小组的Mauchline博士、Penny Hirsch教授和Ian Clark先生也将前往EmbRapa环境进行进一步讨论，并与巴西小组一起起草初步数据的手稿。

The UK team will be comprised of 3 Rothamsted based scientists: Dr. Tim Mauchline (UK Principal Investigator), Prof. Penny Hirsch and Mr. Ian Clark. The Brazilian team from Embrapa environment is composed of three scientists: Dr. Rodrigo Mendes (Brazil Principal Investigator), Dr. Vanessa Nessner and Dr. Maike Rossmann. The complete team have complementary and overlapping skill sets necessary to perform rhizosphere metagenomics and wheat microcosm experiments.UK teamDr. Mauchline is a microbiologist with expertise in rhizosphere microbiology and microcosms of various crops including wheat. Prof. Hirsch is a soil microbiologist with a wealth of experience in soil nutrient cycling. Mr. Ian Clark is a microbiologist with bioinformatics skills. Brazil teamDr. Mendes is a molecular microbiologist with skills in metagenomics of agricultural systems. Dr. Nessner is a molecular biologist with bioinformatics skills. Dr. Maike Rossman a soil microbiologist with experience of wheat cultivation.This pump-priming proposal aims to continue the collaborative links established by the visit of Dr. Tim Mauchline to Dr. Rodrigo Mendes' laboratory at Embrapa Environment for three weeks in May 2014, funded by an ISIS award. Dr. Mauchline's visit proved very useful as it allowed him to become embedded within the Embrapa group. Many discussions were held between Dr. Mauchline and Dr. Mendes, as well with other members of the unit, to determine future directions of microbiome research in the rhizosphere with respect to wheat yields. As a direct result of the visit, a post-doctoral scientist based at Embrapa Environment, Dr. Vanessa Nessner, has joined the Rothamsted team for a period of five months (Dec- May 2015) to work on the wheat microbiome in healthy and diseased plants, and her work will provide a platform for the pump-priming research. Additionally, Dr. Maike Rossmann, who spent a one year post-doctoral position with the Rothamsted team, is now based in Dr. Mendes' laboratory at Embrapa environment working on the metagenomics of the wheat rhizosphere in different soil types. As such, we have developed a functioning team unit between the Rothamsted and Embrapa environment microbiology labs, with complementary skills, which this proposal hopes to build on.The main field experiment has already been setup at Rothamsted, and will require no extra costs as permission has been granted to sample it. The research costs are for soil DNA extractions kits, amplicon sequencing and a comparative pot experiment. Please see the JoR for more information. Wheat sampling, soil DNA extractions and pot study analyses will be a collaborative effort as the visiting Embrapa Brazilian scientists and the Rothamsted team will conduct these together in the Rothamsted field trial and labs during their one month visit to the UK. The main costs requested will be for partners to travel between Brazil and the UK. Dr. Mendes, Dr. Rossmann and Dr. Nessner will travel to the UK to discuss and write the full grant proposal, in addition to performing the collaborative field and lab work described above. We will also invite a further three scientists to provide advice (Prof. Phil Poole and Dr. Gary Bending from the UK, and Dr. Fernando Andreote from Brazil) in a small-scale workshop. It is proposed that Dr. Mauchline, Prof. Penny Hirsch and Mr. Ian Clark from the Rothamsted team will also travel to Embrapa environment in order to further discussions, and to draft a manuscript from the preliminary data with the Brazil team.