BBSRC Embrapa: Temperature resilience of flowering in UK and Brazilian wheat (TempRe)

该项目涉及罗森斯特德研究，诺丁汉大学和Embrapa Trigo。他们之间的团队在非生物胁迫耐受性的遗传控制，花形成的发育遗传学和代谢信号系统的专业知识。他们带来了一系列互补技术，从系统生物学和生物信息学到遗传学和生理学，再到小麦转化。这三个机构都设备齐全，为研究的各个方面提供了设施。目的是利用分子、整株和转基因方法研究英国和巴西小麦对孕穗和开花之间温度胁迫的反应。作物代谢调节遗传学方面的专业知识，以及非生物胁迫如何影响代谢，使用从代谢网络的数学建模到信号传导因子的分子和生物化学分析到RNAseq和GC的技术，MS分析。2013年访问Embrapa Trigo。Matthew Paul博士，Res：在海藻糖6-磷酸信号传导、糖信号传导在响应非生物胁迫中的作用以及在胁迫条件下提高产量的操作方面的专业知识。使用一系列分子和生理技术，以及创新的化学。Huw Jones教授，RRES：在RRes领导小麦转化，利用基因上调，RNAi沉默和基因组编辑，并验证30种不同的基因启动子。2011年参加了BBSRC/Embrapa小麦改良研讨会，并将于2015年在第十五届巴西植物生理学大会上发表全体演讲。诺丁汉大学Zoe Wilson教授：植物发育遗传学方面的专业知识，特别是花粉和花药发育的调控。目前的研究包括将花粉基因网络从模式转化为小麦和大麦，以及拟南芥、小麦和班巴拉花生等作物花粉发育过程中的温度胁迫，这些作物表现出对极端温度的耐受性。诺丁汉大学John Foulkes教授：小麦耐胁迫性状的专业知识。目前的研究包括高通量遥感表型的发展，穗育性的生理和遗传基础以及控制分蘖，小穗原基数量和小花育性的新基因的鉴定。2013年访问Embrapa Trigo。巴西团队：Dr. Embrapa Trigo：在小麦育种方面的专业知识，特别是数量遗传学，经典育种技术，G x E和植物/病原体相互作用，重点是评估和选择植物的非生物胁迫耐受性。Antonio Nhani Jr博士，Embrapa Trigo：生物信息学专业知识应用于分子标记的鉴定和与生物和非生物胁迫相关的小麦转录组数据的分析。tumefaciens介导的小麦遗传转化方案，以产生对非生物胁迫（包括热）具有增加的耐受性的品系。豪尔赫·费尔南多佩雷拉博士：在小麦和大麦的非生物胁迫反应的分子基础方面的专业知识;也对开发小麦和大麦转化技术感兴趣。Luciano Consoli博士：分子遗传学和小麦预育种的专业知识;利用关联遗传学和QTL研究调查非生物胁迫反应和收获前发芽。Mauro塞萨尔Celaro特谢拉博士：在恶劣环境下小麦品种的胁迫耐受性和产量潜力的机制方面的专业知识。Osmar Rodrigues博士：植物生理学方面的专业知识;目前正在研究巴西南部冬季生长季节小麦产量形成和结实的生理基础。

The project involves Rothamsted Research, University of Nottingham, and Embrapa Trigo. Between them the teams have expertise in the genetic control of abiotic stress tolerance, developmental genetics of flower formation, and metabolic signalling systems. They bring a range of complementary techniques, from systems biology and bioinformatics through genetics and physiology to wheat transformation. All three institutions are well-equipped, with facilities for all aspects of the study in place. The aim is to study the response of UK and Brazilian wheat to temperature stress between booting and flowering using molecular, whole-plant and transgenic approaches.UK team:Prof. Nigel Halford, RRes: Expertise in the genetics of metabolic regulation in crop plants and how metabolism is affected by abiotic stress, using techniques ranging from mathematical modelling of metabolic networks through molecular and biochemical analyses of signalling factors to RNAseq and GC-MS analyses. Visited Embrapa Trigo in 2013.Dr Matthew Paul, RRes: Expertise in trehalose 6-phosphate signalling, the role of sugar signalling in responses to abiotic stresses, and manipulation to improve yield under stress conditions. Uses a range of molecular and physiological techniques, as well as innovative chemistry. Prof Huw Jones, RRes: Leads wheat transformation at RRes, exploiting gene up-regulation, RNAi silencing and genome editing and validating 30 different gene promoters. Participated in the BBSRC/Embrapa workshop on wheat improvement in 2011 and will deliver a plenary lecture in the XV Brazilian Congress of Plant Physiology in 2015. Has experience of GMO risk assessment and GM field management.Professor Zoe Wilson, University of Nottingham: Expertise in plant developmental genetics, specifically the regulation of pollen and anther development. Current research involves the translation of pollen gene networks from models to wheat and barley, and temperature stress during pollen development in Arabidopsis, wheat and crops such as Bambara Ground Nut, which show tolerance to extreme temperatures.Prof John Foulkes, University of Nottingham: Expertise in the traits determining stress tolerance in wheat. Current research includes the development of high-throughput remote sensing phenotyping, the physiological and genetic basis of ear fertility and the identification of novel genes controlling tillering, spikelet primordia number and floret fertility. Visited Embrapa Trigo in 2013.Brazil team: Dr Edina Moresco. Embrapa Trigo: Expertise in wheat breeding, specifically quantitative genetics, classical breeding techniques, G x E and plant/pathogen interactions, with a focus on evaluation and selection of plants for abiotic stress tolerance. Dr Antonio Nhani Jr, Embrapa Trigo: Expertise in bioinformatics applied to the identification of molecular markers and the analysis of wheat transcriptome data related to biotic and abiotic stresses.Dr Elene Yamazaki Lau: Expertise in molecular biology and biotechnology; currently developing A. tumefaciens-mediated genetic transformation protocols for wheat in order to generate lines with increased tolerance to abiotic stresses, including heat. Dr Jorge Fernando Pereira: Expertise in the molecular basis of abiotic stress responses in wheat and barley; also has interests in developing wheat and barley transformation techniques. Dr Luciano Consoli: Expertise in molecular genetics and wheat pre-breeding; investigating abiotic stress responses and pre-harvest sprouting using association genetics and QTL studies.Dr Mauro Cesar Celaro Teixeira: Expertise in the mechanisms underpinning stress tolerance and yield potential of wheat cultivars in harsh environments.Dr Osmar Rodrigues: Expertise in plant physiology; currently studying the physiological bases for yield formation and grain set on wheat during the winter growing season in the Southern part of Brazil.Aretha Arcen P. Correa, PhD student