CYTOLOGY AND CONNECTIONS OF THE AUDITORY SYSTEM

这项工作的长期目标是提供对 哺乳动物听觉系统的神经元成分，连同 了解他们的组织和职能。其中一种方法是 为了达到这个目标，我们要识别不同的神经细胞类型。 根据它们特有的细胞化学性质，如 免疫细胞化学。免疫细胞化学鉴定的连接 细胞是通过结合路径跟踪实验和 免疫染色。这样的结果揭示了更多令人担忧的联系 单个细胞类型的数量要比其他情况下更多。抗体 被证明对分析动物组织有用，应用于人类 了解动物和人类组织在多大程度上 类似性，并为推断 人类系统。拟议工作的重点是组织 耳蜗核及其联系。免疫细胞化学将是 用于展示星形藻属的特征细胞化学成分 细胞及其上的轴突终末。独特的化学特征将是 用于区分不同的星状子类。的预测 化学上不同的CN细胞类别将通过注入 辣根过氧化物酶(HRP)进入下丘和外侧 猫丘脑核逆行标记CN神经元 对标记的CN神经元进行免疫染色以显示其特征 化学特征。对CN的输入将以类似方式标识为 将HRP注射到CN，然后逆行进行免疫组织化学染色 上橄榄和下丘内的标记细胞 了解发现了哪些神经肽或神经递质相关酶 在提供CN输入的ELL内。关于现场和现场的问题 章鱼中央核细胞和内侧核主细胞的数量 人类中的梯形身体将使用抗体 描述猫体内这些细胞的特征。结果应该会产生可观的效果 关于脑干的组成和组织的新信息 猫和人类的听觉结构。这样的信息最终会 有助于理解这些结构在听力和睡眠中的作用 为了更好地理解当这些情况发生时出现的赤字的成熟 建筑物有病变或损坏。

The long-term objective of this work is to provide a description of the neuronal elements of the mammalian auditory system, together with knowledge of their organization and function. One of the approaches toward this objective is to identify distinctive neuronal cell classes by their characteristic cytochemical properties, as revealed by immunocytochemistry. Connections of immunocytochemically identified cells are demonstrated by combining pathway tracing experiments with immunostaining. Such results reveal far greater concerning connections of individual cell types than would otherwise be possible. Antibodies that prove useful for analyzing animal tissue are applied to human material to learn the extent to which animal and human tissue are similar and to provide a basis for inferring the organization of the human system. The proposed work focuses on the organization of the cochlear nucleus (CN) and its connection. Immunocytochemistry will be used to demonstrate characteristic cytochemical constituents of stellate cells and of axon terminals on them. Unique chemical signatures will be used to distinguish various stellate subclasses. The projections of chemically distinct CN cell classes will be determined by injecting horseradish peroxidase (HRP) into the inferior colliculus and lateral lemniscal nuclei of cats to retrogradely label CN neurons and then immunostaining the labeled CN neurons to show their characteristic chemical signatures. Inputs to the CN will be similarly identified by injecting HRP into the CN and then immunostaining the retrogradely labeled cells within the superior olive and the inferior colliculus to learn what neuropeptides or neurotransmitter-related enzymes are found within ells providing CN inputs. Issues concerning the presence and numbers of CN octopus cells and principal cells of the medial nucleus of the trapezoid body in humans will be addressed using antibodies that characterize these cells in cats. Results should produce considerable new information about the composition and organization of brainstem auditory structures in cats and humans. Such information will ultimately be useful for understanding the role of these structures in hearing and in better understanding the mature of deficits that occur when these structures are diseased or damaged.