PLACENTAL TRANSFER OF FLUORIDE

产前给药的临床和生物学效应 氟化物在后代中预防龋齿的努力并不是 已定义。尽管许多研究探索了母体-胎儿氟化物 胎儿硬组织中氟的代谢和摄取，数据如下 价值有限，因为与较旧的 在这类研究中使用的氟化物分析。只有两项相关研究使用了 目前公认的检测方法，其中一种是血浆氟 比合理水平高出一个数量级的水平。 此外，一种实用的、相关的、相对便宜的模型动物 尚未确定在哪些系统上进行适当的对照实验 是可以进行的。 我们建议使用豚鼠作为模型系统，因为：(A)牙齿 釉质在产前形成；(B)胎盘在组织上类似于 (C)可以采集足够的胎儿血浆、骨骼和釉质样本； (D)多胎允许连续分娩和取样；(E)几内亚 猪只相对便宜；及。(六)我们已进行所需的 用豚鼠进行的初步研究。 我们开发了一种改进的微扩散氟化法 这使我们能够测量明显较低的胎儿血浆氟水平 (大于0.01ppm)准确。这样的方法对于 母婴血浆氟关系的研究 该项目有两个具体目标：(1)确定短期效果 不同剂量口服氟对母体和胎儿血浆氟的影响 对豚鼠胎牙釉质中氟摄取的影响。以及，(2)至 测定母体和胎儿的血浆氟水平，以及 豚鼠胎牙釉质和骨骼中的浓度维持在 妊娠期的各种氟化物疗法。

The clinical and biological effects of the prenatal administration of fluorides in an effort to prevent dental caries in the offspring are not defined. Although numerous studies have explored maternal-fetal fluoride matabolism and the uptake of fluoride in fetal hard tissues, the data are of limited value because of analytical errors associated with the older fluoride assays used in such studies. Only two relevant studies have used currently accepted assays methods, and one of these reports plasma fluoride levels that are an order of magnitude higher than is reasonable. Furthermore, a practical, relevant and relatively inexpensive model animal system has not been identified on which appropriate controlled experiments can be conducted. We propose to use the guinea pig as a model system because: (a) tooth enamel forms prenatally; (b) the placenta is histologically similar to the human; (c) adequate fetal plasma, bone and enamel samples can be collected; (d) multiple fetuses allow for serial delivery and sampling; (e) guinea pigs are relatively inexpensive; and (f) we have conducted the necessary preliminary studies using the guinea pig. We have developed a modification of the micro-diffusion fluoride method which allows us to measure the apparently low fetal plasma fluoride levels (greater than .01 ppm) with accuracy. Such a method is crucial to the study of maternal-fetal plasma fluoride relationships. The project has two Specific Aims: (1) To determine the short-term effects of selected oral doses of fluoride on maternal and fetal plasma fluoride levels, and on fluoride uptake in fetal enamel in guinea pigs. And, (2) to determine the maternal and fetal plasma fluoride levels, and the fluoride concentrations in fetal enamel and bone in guinea pigs maintained on various fluoride regimens during gestation.