FLAVIN-PROTEIN INTERACTIONS IN FLAVOENZYME CATALYSIS

这个项目的长期目标是阐明结构- 电子转移反应的函数关系 可溶性和膜结合的氧化还原蛋白。 反应 这种类型是一些最重要的基础， 生物学的基本过程，包括呼吸和 固氮，并且是几乎所有代谢的一部分， 系统. 电子转移也与肿瘤学有关， 毒理学和衰老过程我们的方法利用快速 动力学技术（停流分光光度法和激光 闪光光解）作为空间和静电的探针 电子转移位点的性质在各种各样的 具有黄素、血红素、铁硫和铜氧化还原的系统 中心. 具体目标包括电子转移的研究 在溶液中的两个蛋白质部分之间，在静电中， 稳定的1：1复合物，在多亚基蛋白质和蛋白质- 脂质双层组装体。 要研究的一些系统是 黄素-细胞色素c，细胞色素c-细胞色素 过氧化物酶和铁氧还蛋白-铁氧还蛋白还原酶复合物， 黄细胞色素如对甲酚甲基羟化酶， 三甲基胺脱氢酶（一种铁-硫黄素蛋白）， 细菌光合反应中心，细胞色素P450 系统和细胞色素c-细胞色素氧化酶系统。

The long-range goal of this project is to elucidate structure- function relationships for electron transfer reactions involving both soluble and membrane-bound redox proteins. Reactions of this type are the basis for some of the most important and fundamental processes in biology, including respiration and nitrogen fixation, and are a part of virtually all metabolic systems. Electron transfer has also been implicated in oncology, toxicology and the aging process. Out approach utilizes fast kinetic techniques (stopped-flow spectrophotometry and laser flash photolysis) as a probe of the steric and electrostatic properties of the electron transfer site in a wide variety of systems which have flavin, heme, iron-sulfur and copper redox centers. Specific goals involve the study of electron transfer between two protein moieties in solution, in electrostatically- stabilized 1:1 complexes, in multi-subunit proteins and in protein- lipid bilayer assemblies. Some of the systems to be studied are the flavodoxin-cytochrome c, the cytochrome c-cytochrome peroxidase and the ferredoxin-ferredoxin reductase complexes, flavocytochromes such as p-cresol methylhydroxylase, trimethylamine dehydrogenase (an iron-sulfur flavoprotein), bacterial photosynthetic reaction centers, the cytochrome P450 system and the cytochrome c-cytochrome oxidase system.