INHIBITION OF HUMAN CARIOGENIC STREPTOCOCCI

使用具有良好特性的、凝集的、定向的单抗 针对各种表面成分，非凝集突变体 将分离到变形链球菌GS-5。其中将有变种人 包括血清型c抗原在内的一系列表面成分有缺陷， 脂磷壁酸、葡萄糖基转移酶(S)等表面抗原。在……里面 此外，还将尝试分离出有缺陷的GS-5微生物 乳酸脱氢酶。来自现有基因库的质粒库 (在大肠杆菌中产生，由插入嵌合体的GS-5 DNA组成 能够在大肠杆菌和链球菌中复制的质粒)将被使用 将每个缺失的GS-5突变体转化为正常的表面功能。这个 补充功能将通过以下方式进行检测、丰富和选择 用前面提到的单抗进行凝集。一个 替代方案，使用转座子失活来产生两个突变体 和克隆的回复变种，并被认为对 乳酸脱氢酶缺陷菌的分离。该项目是 旨在提供特征良好的、真正等磁学的GS-5对 各种性质有缺陷的衍生品被认为与 致龋性。毒力的体外检测将包括对 疏水组分之间的相互作用，凝集素-配体相互作用， 粘附性多糖的形成和细菌-细菌 互动。我们还计划与其他机构建立合作研究 对这些等基因对进行活体动物研究的调查人员。

Using well characterized, agglutinating, monoclonal antibodies directed against various surface constituents, non-agglutinating mutants of Streptococcus mutans GS-5 will be isolated. Among these will be mutants defective in a range of surface constituents including serotype c antigen, lipoteichoic acid, glucosyl transferase(s), and other surface antigens. In addition, attempts will be made to isolate GS-5 organisms defective in lactic dehydrogenase. Plasmids from an already available gene bank (generated in E. coli and consisting of GS-5 DNA inserts into a chimeric plasmid capable of replication in E. coli and in Streptococci) will be used to transform each deficient GS-5 mutant to normal surface function. The complemented function will be detected, enriched and selected by agglutination using the previously mentioned monoclonal antibodies. An alternate protocol, using transposon inactivation to generate both mutants and cloned revertants, is provided and is considered especially useful for the isolation of lactic dehydrogenese deficient organisms. The project is designed to provide well characterized, truly isogneic pairs of GS-5 derivatives defective in various properties thought to be associated with cariogenicity. In vitro assays for virulence will include studies of interactions between hydrophobic constitutents, lectin-ligand interactions, formation of adhesive polysaccharides, and bacterial-bacterial interactions. We also plan to establish collaborative studies with other investigators for in vivo animal studies of these isogenic pairs.