Novel silver nanoparticles as antibacterial agents

作为抗菌剂的新型银纳米颗粒

• 批准号: 521908-2018
• 负责人: Wilkinson, Kevin
• 金额: $ 2.53万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=699387
• 关键词: Novel; silver; nanoparticles; antibacterial; agents

We aim to extend the advantages of a low-cost technique for the environmentally friendly synthesis of bactericidal silver nanoparticles (Ag NPs). Ag NPs, with unique properties, designed deliberately for specific problems in the health care field will be produced using different types of plant extracts, essential oils and natural antibacterials. The unique synthesis protocol developed by our partner produces different sizes and morphologies of Ag(0) nanoparticles. The goal is to identify the conditions that will give the best synergies between the natural antibacterial coatings and silver nanoparticles, with the goal of developing products that have a unique capacity to eradicate drug resistant bacteria. We will investigate the antibacterial efficacy of these products, by testing their ability to kill several biofilm forming bacterial strains including: Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. We aim to gain detailed insight into the complex action of Ag NP on the bacteria, with the long-term goal of incorporating them into hydrogel scaffolds for the treatment of chronic diseases and tissue regeneration. Nanoparticles will be rigorously characterized using Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), Single Particle Inductively Coupled Plasma Mass Spectroscopy (SP-ICP-MS) and Single Cell Inductively Coupled Plasma Mass Spectroscopy (SC-ICP-MS).

我们的目标是扩大的优势，一个低成本的技术，对环境友好的合成杀菌银纳米粒子（银纳米粒子）。具有独特性质的Ag NPs，专门针对医疗保健领域的特定问题而设计，将使用不同类型的植物提取物，精油和天然抗菌剂生产。我们的合作伙伴开发的独特合成方案可生产不同尺寸和形态的Ag（0）纳米颗粒。我们的目标是确定天然抗菌涂层和银纳米颗粒之间最佳协同作用的条件，目的是开发具有独特能力的产品，以消除耐药细菌。我们将通过测试这些产品杀死几种生物膜形成细菌菌株的能力来研究这些产品的抗菌功效，包括：变形链球菌、大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌。我们的目标是详细了解Ag NP对细菌的复杂作用，长期目标是将它们纳入水凝胶支架中，用于治疗慢性疾病和组织再生。将使用动态光散射（DLS）、透射电子显微镜（TEM）、单颗粒电感耦合等离子体质谱（SP-ICP-MS）和单细胞电感耦合等离子体质谱（SC-ICP-MS）对纳米颗粒进行严格表征。