Novel silver nanoparticles as antibacterial agents

作为抗菌剂的新型银纳米颗粒

• 批准号: 521908-2018
• 负责人: Wilkinson, Kevin
• 金额: $ 2.53万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=719533
• 关键词: Novel; silver; nanoparticles; antibacterial; agents

We aim to extend the advantages of a low-cost technique for the environmentally friendly synthesis of bactericidal silver nanoparticles (Ag NPs). Ag NPs, with unique properties, designed deliberately for specific problems in the health care field will be produced using different types of plant extracts, essential oils and natural antibacterials. The unique synthesis protocol developed by our partner produces different sizes and morphologies of Ag(0) nanoparticles. The goal is to identify the conditions that will give the best synergies between the natural antibacterial coatings and silver nanoparticles, with the goal of developing products that have a unique capacity to eradicate drug resistant bacteria. We will investigate the antibacterial efficacy of these products, by testing their ability to kill several biofilm forming bacterial strains including: Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. We aim to gain detailed insight into the complex action of Ag NP on the bacteria, with the long-term goal of incorporating them into hydrogel scaffolds for the treatment of chronic diseases and tissue regeneration. Nanoparticles will be rigorously characterized using Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), Single Particle Inductively Coupled Plasma Mass Spectroscopy (SP-ICP-MS) and Single Cell Inductively Coupled Plasma Mass Spectroscopy (SC-ICP-MS).

我们的目的是扩展低成本技术的优势，以使杀菌银纳米颗粒（AG NPS）的环保合成。具有独特特性的AG NP是故意为医疗保健领域的特定问题而设计的，将使用不同类型的植物提取物，精油和天然抗菌作品产生。由我们的合作伙伴开发的独特综合方案产生了Ag（0）纳米颗粒的不同大小和形态。目的是确定将在天然抗菌涂层和银纳米颗粒之间提供最佳协同作用的条件，以开发具有独特能力消除耐药细菌的产品。我们将通过测试它们杀死几种生物膜形成细菌菌株的能力，包括：链球菌突变，大肠杆菌，铜绿假单胞菌和金黄色葡萄球菌。我们旨在详细了解Ag NP对细菌的复杂作用，其长期目标是将它们纳入水凝胶支架中，以治疗慢性疾病和组织再生。纳米颗粒将使用动态光散射（DLS），透射电子显微镜（TEM），单个颗粒电感耦合等离子体质谱（SP-ICP-MS）和单个细胞电感偶联的血浆质谱（SC-ICP-MS）来严格表征。