EXTRINSIC CONTROL OF GASTROINTESTINAL MOTILITY

尽管外周自主神经节在维持脑组织的功能中起重要作用 正常胃肠功能，神经节细胞的作用机制 神经节和神经节之间的兴奋性、传递和相互联系 到目前为止，人们对它们的靶器官还没有完全了解。整体而言 这项建议的目的是：(1)阐明在豚鼠、小鼠和 猫，椎前神经节控制运动的机制范围 结肠的活动；以及(2)基本的电生理基础 胰腺内神经节的兴奋性和化学传递 到目前为止胃肠病学中尚未探索的领域。在调查中 肠系膜下神经节(IMG)旨在回答以下问题 问题。首先，做脊髓传入辣椒素的轴突侧支- 敏感神经通过以下方式调节外周内脏感觉-自主神经反射 结肠扩张时IMG释放SP-、NKA-和CGRP-LI？ 第二，中枢神经节前肽是否含有神经突触 IMG门扩张诱导SP-、NKA-和CGRP-LI？第三，做好 结肠肌间VIP能神经元向交感神经元投射 在结肠扩张过程中IMG释放VIP？第四，什么是 含有GABA的结肠传入神经的功能重要性？第五， 结肠机械感受器单位的性质是什么？ 外周反射活动及其在结肠壁的位置？ 对胰腺内神经节的研究旨在回答 以下是问题。首先，在电生理方面有区别吗？ 胰头尾部胰内神经元的特征 胰腺的区域？第二，CCK肽、VIP和5-羟色胺是否参与 在胰腺内突触事件中？第三，是胃十二指肠 将轴突投射发送到胰腺内的肌间神经元 神经节因扩张和/或腔内酸度而激活？细胞内 电生理技术将与放射免疫学相结合， 层析和定量放射密度测量薄层 层析技术。人们希望这些研究能让我们明白 关于人类结肠动力障碍的研究。功能的存在 外周自主神经节中的GABA受体很可能 像苯二氮类这样的抗焦虑药物很可能会影响 患者的胃肠动力。胰腺内神经节的研究 将使我们更好地理解它们的生理重要性 从而为移植的胰腺功能提供了深入的了解 管风琴。

Although peripheral autonomic ganglia are important in the maintenance of normal gastrointestinal functions, the mechanisms of ganglion cell excitability, transmission and interconnectedness between the ganglia and their target organs are as yet imperfectly understood. The overall objectives of this proposal are: (1) to elucidate in guinea pigs, mice and cats, the range of mechanisms by which prevertebral ganglia control motor activity of the colon; and (2) the basic electrophysiology underlying excitability and chemical transmission in intrapancreatic ganglia, a hitherto unexplored area of gastroenterology. Investigations in the inferior mesenteric ganglion (IMG) are designed to answer the following questions. First, do axon collaterals of spinal afferent capsaicin- sensitive nerves mediate a peripheral, visceral sensory-autonomic reflex by releasing SP-, NKA- and CGRP-LI in the IMG during colonic distension? Second, do central preganglionic peptide containing nerves synapsing in the IMG gate distension-induced release of SP-, NKA- and CGRP-LI? Third, do colonic myenteric VIP-containing neurons projecting to sympathetic neurons in the IMG release VIP during colonic distension? Fourth, what is the functional importance of GABA-containing colonic afferents? And, fifth, what is the nature of the colonic mechanoreceptor unit which mediates peripheral reflex activity and where is it located in the colon wall? Investigations in intrapancreatic ganglia are designed to answer the following questions. First, are there differences in electrophysiological characteristics between intrapancreatic neurons in the head and tail regions of the pancreas? Second, do CCK peptides, VIP and 5-HT participate in intrapancreatic synaptic events? And third, are gastroduodenal myenteric neurons that send their axonal projections to intrapancreatic ganglia activated by distension and/or intraluminal acidity? Intracellular electrophysiological techniques will be combined with radioimmunological, chromatographic and quantitative radiodensitometric thin-layer chromatographic techniques. It is hoped that these studies will shed light on disorders of colonic motility in humans. The presence of functional GABA receptors in peripheral autonomic ganglia makes it very likely that anxiolytic drugs like the benzodiazepines very likely affect gastrointestinal motility in patients. Studies on intrapancreatic ganglia will provide a better understanding of their physiological importance thereby providing insights into pancreatic function of the transplanted organ.