EFFECT OF DIET ON ENERGY METABOLISM

肥胖（ob/ob）小鼠和VMH病变大鼠即使成对喂养也会变得肥胖 各自的控制。 因此，这些动物必须减少 比正常的能量。 我们的总体目标是确定 在肥胖动物中提高饮食能量保留的效率 并了解这些过程的调节是如何改变的。 我们 工作假设是骨骼肌中Na+，K+ ATP酶和低Na+，K+ ATP酶的低 棕色脂肪组织的热量输出有助于高保留 膳食能量，以及对后者的主要研究方向 过程 日粮组成和环境温度对 交感神经系统活动，如去甲肾上腺素所示 周转，在棕色脂肪组织和选定的其他器官的瘦肉和 将检查肥胖动物。 因为热原素似乎是 棕色脂肪组织中的一种独特蛋白质，可产生大量热量 在交感神经刺激后，我们还将分析其可用性 通过-3 H-GDP结合棕色脂肪组织线粒体中的蛋白质 procedure. 肾上腺皮质酮和肾上腺儿茶酚胺的作用 在诱导摄食过多，控制骨骼肌Na+，K+ ATP酶方面， 肌肉和棕色脂肪组织中的代谢， 被评价。 肥胖（ob/ob）小鼠和大鼠下丘脑的比较 病变应有助于确定关键的代谢事件， 保持这些动物的饮食能量。 这些数据应该会增加 我们对肥胖症发展的代谢基础的理解， 应该帮助我们开发更好的营养方法来科普 预防和控制肥胖。

Obese (ob/ob) mice and rats with VMH-lesions become obese even if pair-fed to their respective controls. These animals must therefore expend less energy than normal. Our overall goal is to identify mechanisms responsible for the increased efficiency of dietary energy retention in obese animals and to understand how regulation of these processes is altered. Our working hypothesis is that low Na+, K+ ATPase in skeletal muscle and low heat output by brown adipose tissue contribute to the high retention of dietary energy, with the major thrust of the porposed studies on the latter process. Effects of diet composition and environmental temperature on sympathetic nervous system activity, as indicated by norepinephrine turnover, in brown adipose tissue and selected other organs of lean and obese animals will be examined. Because thermogenin appears to be the unique protein in brown adipose tissue that permits high heat production upon sympathetic stimulation we will also assay the availability of this protein in brown adipose tissue mitochondria by the -3H-GDP binding procedure. The role of adrenal corticosterone and adrenal catecholamines in induction of hyperphagia, in control of Na+, K+ ATPase in skeletal muscle and in brown adipose tissue metabolism in obese mice and rats will be evaluated. Comparisons of obese (ob/ob) mice and rats with hypothalamic lesions should help identify key metabolic events that permit high retention of dietary energy in these animals. These data should increase our understanding of the metabolic basis for development of obesity and should aid us in developing improved nutritional approaches to cope with the prevention and control of obesity.