TRANSVERSE NOE IN BIOMACROMOLECULES

研究了自旋锁定态的偶极弛豫现象， 将探索复杂生物分子中的自旋1/2核。 交叉- 松弛过程将产生一个家庭的奥弗豪泽 这些效应可用于阐明结构， 生物分子在溶液中的构象。 我们将尝试 设计产生纯静态锁定的脉冲序列 状态，因此，干扰双量子和哈特曼-哈恩 影响被抑制。 密度矩阵方法将用于 分析复杂自旋系统的行为， 施加的r.f.锁定字段， 磁偶极弛豫 所有的理论预测和 将使用刚性模型对方案进行实验检查 已知结构的化合物。 开发的方法将 应用于生物分子构象的研究， 溶液

The phenomenon of dipolar relaxation in spin-locked states of spin-1/2 nuclei in complex biomolecules will be explored. Cross- relaxation processes will give rise to a family of Overhauser effects which can be useful in the elucidation of structure and conformation of biomolecules in solutions. We will attempt to design pulse sequences giving rise to pure stationary locked states, so that interfering double-quantum and Hartmann-Hahn effects are suppressed. Density matrix methods will be used to analyze the behavior of complex spin systems under the simultaneous influence of an applied r.f. locking field and magnetic dipolar relaxation. All theoretical predications and protocols will be checked experimentally using rigid model compounds of known structures. The methods developed will then be applied to the investigation of conformation of biomolecules in solution.