The development of a phage food additive with the aim to control Salmonella in swine and poultry

研究噬菌体治疗在急性非伤寒沙门氏菌病中作用的动物实验设计共同研究者：Parameth Thiennimitr博士，医学博士，Ph.D.Address:清迈大学医学院微生物学系动物种属：6-8周龄雌性小鼠（Mus musculus）C57 BL 6品系方案（简称）：C57 BL/6小鼠购自Nomura Siam International。小鼠将在实验日前适应至少1周。在肠道鼠伤寒沙门氏菌（菌株IR 715）感染前24小时，小鼠将口服管饲20 mg硫酸链霉素，以允许随后的STM IR 715建立结肠炎。链霉素处理的小鼠将用100微升109 cfu/ml STM IR 715溶液口服感染。然后，将100微升沙门氏菌噬菌体溶液连续3天口服管饲感染的小鼠。在感染后第4天，将通过CO2和颈椎脱位对小鼠实施安乐死。将收集小鼠结肠内容物、结肠、脾脏用于进一步分析。对照组（未处理）小鼠将口服生理盐水溶液作为实验（噬菌体治疗）组的载体。动物方案批准委员会：在清迈大学进行的所有动物实验将由清迈大学动物护理和使用委员会（CMU-ACUC）批准。所有动物实验将在位于清迈大学的AAALAC认证的生物安全等级（BSL）2设施中进行。每组动物数量为7只。[计算如下伯纳德，R。（2000年）的第10/2000号决议。生物统计学基础（第5版）（艾德）。Duxbery：Thomson学习，308，显著性水平= 0.05）]

Animal experimental design to study the role of phage therapy in acute-non typhoidal SalmonellosisCo-investigator: Dr. Parameth Thiennimitr, M.D.,Ph.D.Address: Department of Microbiology, Faculty of Medicine, Chiang Mai UniversitySpecies of animal: 6-8 weeks old female mouse (Mus musculus) C57BL6 strainProtocol (in brief): C57BL/6 mice will be purchased from Nomura Siam International. Mice will be acclimatized for at least 1 week before the experiment day. 24 hour before Salmonella enterica Typhimurium (strain IR715) infection, mouse will be orally gavaged with 20 mg streptomycin sulphate to allow subsequent STM IR715 to establish a colitis. Streptomycin-treated mice will be orally infected with 100 microlitr of 109 cfu/ml STM IR715 solution. Then, 100 microlitr of Salmonella phage solution will be orally gavaged to the infected mice for consecutively 3 days. On day 4 post infection, mice will be euthanized by CO2 and cervical dislocation. Mouse colon content, colon, spleen will be collected for further analysis. Control group (no treatment) of mice will be orally fed with normal saline solution as used as a vehicle for the experimental (phage therapy) groups.Animal protocol approval committee: All animal experiments conduct at Chiang Mai University will be approved by the Chiang Mai University Animal Care and Use Committee (CMU-ACUC). All animal experiments will be performed in the AAALAC-certified biosafety level (BSL) 2 facility located in Chiang Mai University. Numbers of animal in each group is 7. [Calculated followed Bernard, R. (2000). Fundamentals of biostatistics (5th ed). Duxbery: Thomson learning, 308, level of significance = 0.05)]