OBSTRUCTED BLADDER MUSCLE: ELECTROPHYSIOLOGICAL EFFECTS

膀胱出口梗阻可导致明显的临床排尿 手术解除梗阻后可能持续存在的功能障碍， 在某些情况下，这是不可逆转的。关于以下影响的信息 障碍和解除障碍的时机的影响可能成为 在与临床泌尿外科实践相关的决策政策中很重要 和公共卫生政策，其中临床试验、结果分析和 都涉及到生活质量。相关的临床和实验数据 梗阻对膀胱功能的影响一直在稳步积累。 然而，关于电学变化的信息很少 与障碍物相关的更改的基础属性。这项建议 请求支持利用最近开发的豚鼠模型 研究这些尿路梗阻的电学变化。 在前一次拨款期间，我们建造和装备了 临床尿路平滑肌研究的实验室 泌尿科。我们已经建立了一种渐进性偏爱的动物模型。 用豚鼠研究尿路梗阻的功能和功能 结构性膀胱改变。除了研究其影响之外， 膀胱梗阻及其释放对体内尿流动力学参数的影响 体重、膀胱肌力量发育、胆碱能神经含量和 胶原蛋白含量，该模型已首次成功 无源电学特性的细胞内电生理研究 梗阻的膀胱肌。 目前的建议是为了研究被动和主动的变化。 阻塞物及其释放后的电膜性质 在一个豚鼠模型中。计划中的研究包括测量休息时间 膜电位、长度常数、时间常数、作用变化 潜能型、毒鼠型和非肾上腺素能、非胆碱能神经肌肉 变速箱。与其他功能和结构变化的相关性 将会被执行。该项目的长期目标是产生一个 对障碍及其释放的影响的综合描述 单侧膀胱的功能和结构特征 哺乳动物模型。

Bladder outlet obstruction can produce significant clinical voiding dysfunction which may persist after surgical relief of obstruction and, in some cases, be irreversible. Information regarding the effects of obstruction and the influence of timing of relief of obstruction may become important in decision-making policy related to clinical urologic practices and public health policy where clinical trials, outcome analysis and quality of life are involved. Clinical and experimental data regarding the effects of obstruction on bladder function has been steadily accumulating. However, there has been very little information about changes in electrical properties underlying changes related to obstruction. The proposal requests support to utilize a recently developed guinea pig model of urethral obstruction to study these electrical changes. During the period of the previous grant, we have constructed and equipped a laboratory of the study of urinary track smooth muscle in a clinical urology department. We have developed an animal model of gradual partial urethral obstruction using the guinea pig to study functional and structural bladder changes. In addition to studying the effect of obstruction and its release on in vivo urodynamic parameters, bladder weight, bladder muscle force development, cholinergic nerve content and collagen content, the model has permitted the first successful intracellular electrophysiological studies of passive electrical properties of obstructed bladder muscle. The present proposal is for the study of changes in passive and active electrical membrane properties occurring after obstruction and its release in a guinea pig model. Studies planned include measurement of resting membrane potential, length constant, time constant, changes in action potential and muscarinic and non-adrenergic, non-cholinergic neuromuscular transmission. Correlation with other functional and structural changes will be performed. The long term goal of the project is to produce a comprehensive description of the effects of obstruction and its release on the functional and structural properties of the urinary bladder in a single mammalian model.