OBSTRUCTED BLADDER MUSCLE--ELECTROPHYSIOLOGICAL EFFECTS

膀胱出口梗阻可产生显著的临床排尿 梗阻手术缓解后可能持续存在的功能障碍， 在某些情况下，是不可逆转的。 关于影响的资料 阻塞和阻塞解除时间的影响可能成为 在临床泌尿外科实践相关决策中的重要性 和公共卫生政策，其中临床试验，结果分析和 生活质量是有关系的。 临床和实验数据， 梗阻对膀胱功能的影响一直在稳步积累。 然而，关于电力变化的信息很少。 与阻塞相关的潜在变化的性质。 该提案 请求支持利用最近开发的豚鼠模型， 尿道阻塞来研究这些电变化。 在上一次拨款期间，我们建造并装备了 一个研究泌尿道平滑肌在临床中的实验室 泌尿外科。 我们建立了一种渐进性局部缺血的动物模型， 使用豚鼠研究尿道梗阻的功能和 膀胱结构改变。 除了研究 梗阻及其解除对体内尿动力学参数的影响，膀胱 体重、膀胱肌力发育、胆碱能神经含量和 胶原蛋白含量，该模型已允许第一次成功 被动电特性的细胞内电生理学研究 膀胱肌肉阻塞 目前的建议是研究被动和主动的变化 阻塞后的膜电特性及其释放 在豚鼠模型中。 计划的研究包括测量静息 膜电位，长度常数，时间常数，动作变化 电位和毒蕈碱和非肾上腺素能、非胆碱能神经肌肉 传输 与其他功能和结构变化的相关性 将被执行。 该项目的长期目标是产生一个 全面说明阻塞和解除阻塞对 膀胱的功能和结构特性在一个单一的 哺乳动物模型

Bladder outlet obstruction can produce significant clinical voiding dysfunction which may persist after surgical relief of obstruction and, in some cases, be irreversible. Information regarding the effects of obstruction and the influence of timing of relief of obstruction may become important in decision-making policy related to clinical urologic practices and public health policy where clinical trials, outcome analysis and quality of life are involved. Clinical and experimental data regarding the effects of obstruction on bladder function has been steadily accumulating. However, there has been very little information about changes in electrical properties underlying changes related to obstruction. The proposal requests support to utilize a recently developed guinea pig model of urethral obstruction to study these electrical changes. During the period of the previous grant, we have constructed and equipped a laboratory of the study of urinary track smooth muscle in a clinical urology department. We have developed an animal model of gradual partial urethral obstruction using the guinea pig to study functional and structural bladder changes. In addition to studying the effect of obstruction and its release on in vivo urodynamic parameters, bladder weight, bladder muscle force development, cholinergic nerve content and collagen content, the model has permitted the first successful intracellular electrophysiological studies of passive electrical properties of obstructed bladder muscle. The present proposal is for the study of changes in passive and active electrical membrane properties occurring after obstruction and its release in a guinea pig model. Studies planned include measurement of resting membrane potential, length constant, time constant, changes in action potential and muscarinic and non-adrenergic, non-cholinergic neuromuscular transmission. Correlation with other functional and structural changes will be performed. The long term goal of the project is to produce a comprehensive description of the effects of obstruction and its release on the functional and structural properties of the urinary bladder in a single mammalian model.