ROLE OF LIPIDS IN UROLITHIASIS

描述(改编自申请者摘要)：在美国， 肾结石占每1000家医院中的7%到10% 入院人数和1,000人中有1人患有结石病 在他们有生之年。大约80%的结石含有钙 草酸，尿酸和鸟粪石约占10-15%。这很好 承认在石头萌生过程中的一个重要事件是异质 石晶成核，而这种基质是主要的 有机成核物质的来源。特别值得关注的是 可能是尿液中存在的酸性磷脂 细胞损伤，损伤的细胞排入尿液，细胞 死亡，以及膜成分释放到尿液中。 遵循骨生长领域表达的理论，以及事实 磷灰石形式的磷酸钙经常被发现是 草酸钙结石中的病灶，应用假设为酸性 来自肾小管质膜的磷脂形成复合体 与钙和无机磷酸盐，然后排序，成为磷灰石。 首席调查员和他的同事是唯一的研究人员 小组将探索这一可能性在结石疾病。这是一个重要的 这是一个主题，肯定需要进一步调查。 拟议研究的主要目标是调查是否存在 草酸钙、鸟粪石和尿酸结石中的脂类识别 各种脂类及其在钙成核中的作用 草酸盐晶体的体外实验。实验计划将比较血脂 用在这些结石中发现的脂成分测定这些结石中的成分 肾上皮细胞质膜。这些研究中的假设是 草酸钙结石会含有磷脂，尿酸结石会 磷脂含量较少，鸟粪石(形成于 氨分解细菌的存在)将包含两者的复合体 糖脂和磷脂。

DESCRIPTION (Adapted from the Applicant's Abstract): In the United States, renal stone disease accounts for 7 to 10 out of every 1,000 hospital admissions and 1 of 1,000 individuals are afflicted with stone disease during their lifetime. Approximately 80% of all stones contain calcium oxalate, and uric acid and struvite accounts for about 10-15%. It is well accepted that an important event in stone initiation is the heterogeneous nucleation of stone crystals, and that matrix serves as one of the major sources of organic nucleation materials. Of special interest are the acidic phospholipids which are present in the urine probably as the result of cellular injury, sloughing of the damaged cells into the urine, cell death, and the release of membrane components into the urine. Following the theories expressed in the bone growth field, and the fact that calcium phosphate in the form of apatite is frequently found as the nidus in calcium oxalate stones, the application hypothesizes that acidic phospholipids derived from the renal tubule plasma membrane form a complex with calcium and inorganic phosphate which then orders and becomes apatite. The principal investigator and his colleagues have been the only research group to explore this possibility in stone disease. This is an important subject and definitely requires further investigation. The main goal of the proposed research is to investigate the presence of lipids in calcium oxalate, struvite, and uric acid stones, identify the individual lipids and determine their role in the nucleation of calcium oxalate crystals in vitro. The experimental plan will compare the lipid composition determined in these stones with the lipid composition found in renal epithelial plasma membranes. The hypothesis in these studies is that calcium oxalate stones will contain phospholipids, uric acid stones will contain less phospholipid content, and struvite stones (which are formed in the presence of ammonia-splitting bacteria) will contain both complexed glycolipids as well as phospholipids.