HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS

人们普遍认为，在一定程度上对昆虫进行化学防治 将是维护人类健康和农业生产所必需的 在可预见的未来的生产力。如果我们要满足这些健康和 生产力标准，同时保护环境和人类免受 有潜在危险的农药，我们必须a)开发安全的、可生物降解的 虫害防治药剂，b)了解目标和 以及c)开发快速、灵敏的分析方法 能够检测到潜在危险的外源物质。因此， 该项目的长期目标是保护人类和 通过解决环境卫生方面的上述问题。一、我们会的 开发环境可接受的昆虫防治剂的新线索 探索我们的MOST蜕变中的关键事件 破坏性的害虫。这些线索有望被双方利用 古典化学和基因工程。二、自从环氧化物部分 是一些最危险的毒素、诱变剂和 致癌物质已知，我们将研究其在哺乳动物体内的代谢 系统。将特别注意诱导和抑制 这些系统。使用选择性底物、诱导剂和抑制剂，我们 将致力于开发环氧化物的预测模型 分解代谢。最后，我们将评估免疫化学技术作为一种 能够快速和廉价地分析经典和 创新的杀虫剂。

It is generally accepted that chemical control of insects at some level will be necessary for the preservation of human health and for agricultural productivity in the foreseeable future. If we are to meet these health and productivity standards while protecting the environment and man from potentially dangerous pesticides, we must a) develop safe, biodegradable pest control agents, b) understand xenobiotic metabolism in target and nontarget organisms, and c) develop rapid, sensitive analytical methods capable of detecting potentially dangerous xenobiotics. Thus, the long-term goal of this project is the safeguarding of human and environmental health by solving aspects of the above problems. I. We will develop new leads for environmentally acceptable insect control agents by investigating the critical events in the metamorphosis of our most destructive insect pests. THese leads can hopefully be exploited by both classical chemistry and genetic engineering. II. Since the epoxide moiety is the reactive center in some of the most dangerous toxins, mutagens and carcinogens known, we will investigate its metabolism in mammalian systems. Special attention will be paid to the induction and inhibition of these systems. Using selective substrates, inducers and inhibitors, we will work toward the development of a predictive model of epoxide catabolism. III. Finally, we will evaluate immunochemical technology as a technique capable of rapidly and inexpensively analyzing both classical and innovative pesticides.