16-ERACAPS: Signaling complexes in plant immunity and development "SICOPID"

植物是大自然对多细胞生命的另一个成功实验。为了协调细胞、组织和器官的生长发育，植物进化出了独特的质膜受体激酶（RKs）。该蛋白家族的几个成员作为模式识别受体和激素受体发挥作用，形成植物的结构。越来越多的证据表明，不同的植物RKs被组织在膜信号复合体中。rk具有共同的结构架构，并共享下游信令组件。因此，目前尚不清楚细胞表面对特定内源性或外源性信号的识别如何转化为细胞溶胶中特定发育程序或免疫反应的激活。我们建议将生理学、遗传学和细胞生物学与磷蛋白质组学、定量生物化学和结构生物学相结合，以确定植物发育和免疫受体复合物激活的共同和特异性机制。我们将详细剖析活化的受体复合物如何在细胞质中产生特定的信号输出，以及植物RKs的活性如何受到抑制蛋白的调节。我们设想，我们的工作将为理解RK信号在分子水平上的特异性是如何编码的提供一个分子框架，为将来在作物中设计这些途径奠定基础。

Plants are nature's other successful experiment with multicellular life. To coordinate growth and development of their cells, tissues and organs plants have evolved unique plasma membrane receptor kinases (RKs). Several members of this protein family function as pattern recognition receptors, and as hormone receptors shaping the architecture of the plant. There is mounting evidence that different plant RKs are organized in membrane signaling complexes. RKs have a common structural architecture and share downstream signaling components. As such, it is presently unclear how the recognition of specific endogenous or foreign signals at the cell surface is translated into the activation of specific developmental programs or immune responses in the cytosol. We propose to combine physiology, genetics and cell biology with phosphoproteomics, quantitative biochemistry and structural biology to identify the shared and specific mechanisms by which plant developmental and immune receptor complexes are activated. We will dissect, in molecular detail, how activated receptor complexes generate specific signaling output in the cytosol and how the activity of plant RKs are regulated by inhibitor proteins. We envision that our work will provide a molecular framework for understanding how specificity is encoded at the molecular level in RK signaling, setting the stage for engineering these pathways in crops in the future.