FUNCTIONAL ORGANIZATION OF PRIMATE FRONTAL EYE FIELDS

这些拟议的实验将继续调查 灵长类前额眼场(FEF)的功能组织 它在控制自主眼球运动方面的作用。这 感觉运动皮质的研究将在单细胞、柱状和 行为水平。实验分析了酶的活性。 恒河猴大脑皮质神经元的个体表现 感觉运动测试。录音的补充是视力测试。 通过微刺激引起的运动 记录电极。解剖学上的实验性损伤，以及 代谢研究是对生理学研究的补充。 特定目标损害，调查FEF在平滑中的作用 追踪(SP)眼球运动。FEF的次区域是光滑的， 而不是扫视，眼球运动将被映射并 我们将分析那里的神经元活动与SP的关系。 SP分区的连接将使用HRP进行映射。它的重要性 SP的FEF将通过FEF损害进行评估，包括 通过微量注射神经毒素造成SP区的离散损害。 另一个目标是FEF在眼球跳动中的作用 到听觉目标。听觉活动的地形图横跨 FEF将被映射，以及听觉是否接受的问题 FEF中的场因凝视的改变而被重新映射，将被解决。 听觉重新映射研究与更一般的调查相一致。 进入哪个坐标系，视网膜或颅视，最好 描述与FEF扫视相关的不同类型的活动。 这项调查涉及重新映射FEF的响应字段 来自不同固定点的神经元，并将特别 指向电引起眼球运动的FEF部位 有“目标导向”的轨迹。 一系列目标围绕着 FEF细胞的生理类型，例如它们的板层位置 以及传出的投射。层流位置将通过以下方式解决 在特定细胞类型的位置进行标记沉积。 通过植入体部的电极进行逆行激活 胼胝体将决定每个大脑中的FEF信息 半球发送另一个。FEF的功能地形图 将用双标记2-DG技术进行映射。它的起源 FEF中的视觉活动将通过在那里进行记录来确定 实验性损毁纹状皮质及上皮层后 丘脑。最后，这次调查的范围将是 扩展以提供弓状沟FEF与 恒河猴的其他皮质眼区，即 额叶内侧和顶眼的补充眼野 菲尔兹。 在临床上，这些研究将有助于诊断和治疗 眼球运动障碍通常伴随着神经性和 精神障碍。选举结果将影响到 大脑皮层在指挥眼球运动方面，应该有助于解释 为什么灵长类动物的大脑有多个皮质眼区？ 它们的不同目的和专业是。

These proposed experiments will continue investigations into the functional organization of the primate frontal eye fields (FEF) and its role in the control of voluntary eye movements. This sensorimotor cortex will be studied at single cell, columnar, and behavioral levels. The experiments analyze the activity of individual cortical neurons in rhesus monkeys performing sets of sensorimotor tests. Recordings are supplemented by tests for eye movements electrically elicited by microstimulation through the recording electrodes. Anatomical, experimental lesion, and metabolic studies complement the physiological investigations. Specific objectives lesion, investigating the FEF's role in smooth pursuit (SP) eye movements. The subregion of FEF where smooth, rather than saccadic, eye movements are elicited will be mapped and neuronal activity there will be analyzed in relation to SP. Connections of the SP zone will be mapped with HRP. The importance of the FEF for SP will be assessed with FEF lesions, including discrete lesions of the SP zone via microinjection of neurotoxins. Another objective concerns the FEF's role in saccadic eye movements to auditory targets. The topography of auditory activity across the FEF will be mapped, and the issue of whether auditory receptive fields in FEF are remapped by changes in gaze will be addressed. The auditory remapping study parallels a more general investigation into which coordinate system, retinotopic or craniotopic, best describes the different types of FEF saccade-related activity. This investigation involves remapping the response fields of FEF neurons from different fixation points, and will be especially directed at FEF sites where electrically elicited eye movements have "goal-directed" trajectories. A set of objectives revolves around anatomical correlates of the physiological types of FEF cells, such as their laminar location and efferent projections. Laminar location will be addressed by making marking deposits at the sites of particular cell types. Antidromic activation via electrodes implanted in the corpus callosum will determine what information the FEF in each cerebral hemisphere sends the other. The functional topography of the FEF will be mapped with the double-label 2-DG technique. The origin of visual activity in FEF will be determined by recording there after experimental lesions of striate cortex and superior colliculus. Finally, the scope of this investigation will be expanded to provide a comparison of the arcuate sulcus FEF with other cortical eye fields of the rhesus monkey, namely the supplemental eye fields in the medial frontal lobe and parietal eye fields. Clinically, these studies will help in diagnosis and treatment of the oculomotor disturbances that often accompany neurological and psychiatric disorders. The results will bear on the role of the cerebral cortex in commanding eye movement, and should help explain why the primate brain has multiple cortical eye fields and what their different purposes and specializations are.