CELL BIOLOGY OF THE RETINA

此应用程序涉及将超微结构和 神经研究中的电生理技术 哺乳动物视网膜中的相互作用。它由三个部分组成： I)我们想要从水平和双极感光器记录 从兔视网膜分离的切片和细胞团中的细胞和 用电子显微镜分析它们之间的联系。 这些实验的目的是为了了解 各种类型的双极细胞及其机制 它们的响应特性。Ii)我们想要从神经元记录 保存在超融合眼罩中的猕猴视网膜 可见光细胞内注射法制备及鉴定 记号笔。这些实验的目的是调查 视网膜的功能连接非常类似于人类的视网膜。 三)将4℃快速冷冻、冷冻-压裂、 超微结构示踪技术和电生理学，我们希望 分析递质释放和突触的机制 小鼠孤立光感受器细胞囊泡膜的再循环 兔子的视网膜。此应用程序的长期目标是 发现视网膜处理视觉的机制 信息。这类研究是 对老年人精神障碍的认识和合理治疗 视网膜和神经系统。

This application is concerned with combining ultrastructural and electrophysiological techniques in the study of the neural interactions in the mammalian retina. It consists of three parts: i) We want to record from photoreceptors, horizontal and bipolar cells in slices and cell clumps isolated from the rabbit retina and to analyze their connections with the electron microscope. Purpose of these experiments is to understand the physiology of the various types of bipolar cells and the mechanisms underlying their response properties. ii) We want to record from the neurons of the macaque monkey retina maintained in a superfused eyecup preparation and identify them by intracellular injection of visible markers. Purpose of these experiments is to investigate the functional connectivity of a retina very similar to the human one. iii) By combining rapid freezing at 4 degree K , freeze-fracturing, ultrastructural tracer techniques and electrophysiology, we wish to analyze the mechanism of transmitter release and synaptic vesicle membrane recycling in solitary photoreceptor cells from the rabbit retina. Long-term objective of this application is to discover the mechanisms by which the retina processes visual information. This sort of studies are fundamental to the understanding and rational treatment of the disorders of the retina and nervous system in general.