18-BTT: Induction of double haploids in wheat using CENH3 mutants and genome editing

传统的小麦育种需要六到七代的自花授粉才能产生高度纯合子和稳定的品种。另一种方法是生产双单倍体（DH），这减少了将真正的育种系交付给一代的世代时间。目前小麦DH技术（基于玉米授粉）很费力，需要高水平的专业知识和设备齐全的实验室。这些都是昂贵的资源，即使在发达国家也严重限制了卫生保健的使用。在模式物种拟南芥中，基因CENH3的氨基酸变化导致单倍体诱导（HI）系的产生。我们提出了一种策略，将该基因的突变体和基因编辑版本结合起来，改变小麦的CENH3基因，并开发单倍体诱导系。我们的最终目标是通过开发高效的单倍体诱导系来加速小麦育种，这些单倍体诱导系将产生双倍的单倍体（DH）作为种子

Traditional wheat breeding requires six to seven generations of self-pollination to deliver highly homozygous and stable cultivars. An alternative approach is the production of doubled haploids (DH) which reduces the generation time to deliver true-breeding lines to a single generation. Current DH technologies in wheat (based on maize pollination) are laborious and require a high level of expertise and well-equipped laboratories. These are expensive resources and severely limit DH use even in developed countries. Amino acid changes in a gene called CENH3 result in haploid inducing (HI) lines in the model species Arabidopsis. We propose a strategy to combine mutants and gene-edited versions of this gene to alter CENH3 in wheat and develop haploid inducer lines. Our final goal is to accelerate wheat breeding by developing efficient haploid inducer lines that will produce doubled haploids (DH) as seeds