18-BTT EAGER: A system for the production of haploid inducer lines and cytoplasmic male sterile doubled haploids for efficient hybrid production

杂交品种是现代作物生产的基石。种子公司花费多年时间开发优良的近交亲本系，用于生产农民每个季节种植的杂交种子。植物育种周期的一个主要瓶颈是需要反复回交有前途的亲本系，使其完全近交。一种节省时间的解决方案是使用双单倍体在一代内直接产生完全自交系。用这种方法，一个单倍体植物首先被分离出来，然后用化学处理使其染色体加倍。由此产生的双单倍体根据定义是完全近交的。为了使这种策略对植物育种者有实际的应用，需要一种可靠的方法来产生初始的单倍体植株。在本提案中，我们的目标是开发和测试一种制造单倍体诱导系的一般策略，为植物育种者提供有效生产用于杂交种子生产的双单倍体优质亲本系所需的工具。

Hybrid cultivars are a cornerstone of modern crop production. Seed companies spend many years developing elite inbred parental lines that are used to produce the hybrid seed that farmers plant each season. A major bottleneck in the plant breeding cycle is the need to repeatedly backcross promising parental lines to make them fully inbred. One time-saving solution to this challenge is to use double haploids to directly produce fully inbred lines in one generation. With this approach a haploid plant is first isolated, and then chemical treatment is used to double its chromosomes. The resulting double haploid is by definition fully inbred. For this strategy to be of practical use to plant breeders, a reliable method for producing the initial haploid plants is required. In this proposal our objective is to develop and test a general strategy for making haploid inducer lines that should provide plant breeders with the tool they need to efficiently produce double haploid elite parental lines for hybrid seed production.