18-BTT EAGER: A system for the production of haploid inducer lines and cytoplasmic male sterile doubled haploids for efficient hybrid production

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Hybrid cultivars are a cornerstone of modern crop production. Seed companies spend many years developing elite inbred parental lines that are used to produce the hybrid seed that farmers plant each season. A major bottleneck in the plant breeding cycle is the need to repeatedly backcross promising parental lines to make them fully inbred. One time-saving solution to this challenge is to use double haploids to directly produce fully inbred lines in one generation. With this approach a haploid plant is first isolated, and then chemical treatment is used to double its chromosomes. The resulting double haploid is by definition fully inbred. For this strategy to be of practical use to plant breeders, a reliable method for producing the initial haploid plants is required. In this proposal our objective is to develop and test a general strategy for making haploid inducer lines that should provide plant breeders with the tool they need to efficiently produce double haploid elite parental lines for hybrid seed production.