WOUND HEALING MODULATION IN GLAUCOMA FILTERING SURGERY

这个项目的主要目标是开发新的方法 用于控制青光眼滤过术后的伤口愈合 通过聚合物传递技术的外科手术(GFS)- 有控制的药物输送。可生物侵蚀的聚合物将被用于 多种抗瘢痕药物的局部性和持久性 在动物模型中GFS部位的组织形成。一系列 前瞻性、随机性、双掩蔽性和安慰剂- 在动物模型中进行的对照实验将由 用某种药物浸渍的生物可腐蚀聚合物的比较 或药物组合到相同的生物可腐蚀聚合物中，而没有任何 毒品。所有动物都将接受术前眼科检查。 包括裂隙灯生物显微镜和气体计量学。一个 标准的双眼后唇巩膜切除手术 在全身麻醉下的每只动物。与药物有关的聚合物 会被随机植入一只眼睛，而另一只眼睛 接受安慰剂聚合物。将进行眼睛检查 术后一天，此后每隔一天进行一次 至少五周或直到滤过手术在 实验眼失灵。测量的参数将包括 眼内压，流出设施，滤过泡的出现， 结膜，聚合物植入物，角膜，前房。在… 术后不同时间进行组织病理学检查 将在实验眼和对照眼上进行。 将使用定量形态学和免疫组织化学方法 评价药物给药对瘢痕形成的影响。这项研究将 开发和提炼聚合物组合物的特性， 溶出度、药物释放率、几何形状和 可能与不同的药物层叠，以改善其 GFS的疗效。成纤维细胞体外组织培养体系的建立 将被开发用于筛选潜在有用的药物以供输送 生物可降解聚合物及其药物释放的研究 浸渍生物可腐蚀聚合物的特性研究 毒品。在体外成纤维细胞的生长将被直接测量 成纤维细胞计数和甲基-14C胸腺嘧啶核苷摄取法。 在GFS中使用可生物降解聚合物作为药物输送装置可能 极大地提高了这一手术的成功率 失败风险很高。

The primary objective of this project is to develop new methods for controlling wound healing following glaucoma filtration surgery (GFS) through the technology of polymeric delivery- controlled drug delivery. Bioerodible polymers will be used fot the localized and sustained of various drugs which inhibit scar tissue formation at the site of GFS in an animal model. A series of prospective, randomized, double-masked, and placebo- controlled experiments in an animal model will be performed by comparing a bioerodible polymer impregnated with a certain drug or drug combination to the same bioerodible polymer without any drug. All animals will undergo preoperative eye examinations including slit lamp biomicroscopy and pneumotonomery. A standard posterior lip sclerectomy will be performed on both eyes of each animal under general anesthesia. The polymer with drug will be randomly implanted in one eye and the fellow eye will receive the placebo polymer. Eye examinations will be performed one day after surgery and every other day thereafter for a minimum of five weeks or until the filtering surgery in the experimental eye fails. The parameters measured will include intraocular pressure, outflow facility, the appearance of the bleb, conjunctiva, polymer implant, cornea, and anterior chamber. At various times following surgery, histopathological examination will be performed on both experimental and control eyes. Quantitative morphology and immunohistochemistry will be used to assess the effect of drug delivery on scarring. This study will develop and refine the polymer characteristics of composition, dissolution rate, drug release rate, geometrical shape, and possible lamination with different drugs in order to improve its efficacy in GFS. An in vitro tissue culture system of fibroblasts will be developed to screen potentially useful drugs for delivery by the bioerodible polymers and to study the drug release characteristics of the bioerodible polymers impregnated with drugs. The in vitro fibroblastic growth will be measured by direct counting of fibroblasts and by using methyl-14C thymidine uptake. Using bioerodible polymers as a drug delivery device in GFS may dramatically improve the success of this procedure in patients at high risk for failure.