BIOLOGICAL OXIDATION MECHANISMS

该研究项目的主要目的是了解化学物质 黄素辅酶的反应性以及如何使用这种反应性， 控制在生物系统中。 接近的方法将是 研究代表各种类别的特定酶， 黄素蛋白和模型系统的研究。 将会有大量的使用 稳态动力学与快速反应动力学研究相结合，以及 用一种新的蛋白质替代天然的辅酶， 合成黄素，以测试假设的方案，并探讨 直接围绕黄素的环境的性质。 后者是 在提供有关活性部位的信息方面尤其重要 蛋白质中的一个区域，其结构不能从X射线中获得 结晶学 我们还计划进行的氨基酸序列的 L-乳酸氧化酶，以帮助晶体学测定 它的三维结构。

The main aim of the research project is an understanding of the chemical reactivity of flavin coenzymes and how this reactivity is used and controlled in biological systems. The methods of approach will be the study of specific enzymes representative of the various classes of flavoproteins and studies of model systems. Considerable use will be made of steady state kinetics coupled with rapid reaction kinetic studies, and of the technique of replacing the natural coenzymes of falvoproteins with synthetic flavins, in order to test hypothetical schemes and to probe the nature of the environment immediately around the flavin. The latter is particularly important in providing information about the active site region in proteins where the structure is not available from X-ray crystallography. We also plan to undertake the amino acid sequence of L-lactate oxidase in order to aid in the crystallographic determination of its three-dimensional structure.