BIOMEDICAL SEPARATIONS--FIELD-FLOW FRACTIONATION

本研究计划的长期目标是发展和扩大 场流分馏（FFF）技术，以这种方式，其固有的 这些优点可以容易地实现并应用于复杂的生物化学过程。 材料. 另一个目标是将分离的边界推回 方法论 为实现上述目标，将开展以下四类工作： 1. FFF的一般优化。 在这里，我们将寻求改进， 仪器，通道设计和程序，以使FFF更多 对广大科学界来说更容易获得和更有用。 的一部分 在这项研究中，我们将研究编程FFF的优化。 2.生物化学应用。 我们将直接参与几个项目， 生物化学的相关性，主要与来自 生物医学社区 3.二维FFF。 我们将采取一项重大的长期举措， 联合收割机结合了二维切割机的高分辨率和多功能性， FFF在分离中显示出的优势， 大分子和颗粒成分。 我们希望通过这种方式 新的强大的分离技术。 4.分离科学的发展。 在这里，我们将继续我们的 长期努力，广泛推进理论认识， 基本分离过程。 此外，我们将寻求开发新的 分离方法和技术。

The long term objective of this research proposal is to develop and expand field-flow fractionation (FFF) technology in such a way that its intrinsic advantages can be readily realized and applied to complex biochemical materials. Another objective is to push back the frontiers of separation methodology. The above objectives are to be met by pursuing work in four categories: 1. General Optimization of FFF. Here we weill seek improvements in instrumentation, channel design, and procedures in order to make FFF more accessible and more useful to the broad scientific community. As part of this study, we will examine the optimization of programmed FFF. 2. Biochemical Applications. We will engage in several projects of direct biochemical relevance, working mainly with collaborators from the biomedical community. 3. Two-Dimensional FFF. We will undertake a major long-term initiative to combine the high resolving power and versatility of two-dimensional methodology with the advantage displayed by FFF in the separation of macromolecular and particulate components. In this way we hope to evolve new and powerful separation techniques. 4. Developments in Separation Science. Here we will continue our long-standing efforts to broadly advance the theoretical understanding of basic separation processes. In addition, we will seek to develop new separation approaches and techniques.