Underpinning UK Bioscience Research with high-throughput single molecule sequencing

基因组学革命正在进入第三阶段，技术的出现可以产生扩展到数百千碱基的单分子DNA测序数据。长DNA序列读取可用于生成高质量的参考基因组，以更好地了解基因表达，并在复杂混合物（如土壤或水的环境样品）中对DNA进行测序和排序。太平洋生物科学Sequel II系统是一种新的长读DNA测序平台，以可承受的成本生成大量数据。Sequel II的一个关键特点是，它可以通过多次读取DNA分子来纠正错误，从而产生高度准确的长读。这笔拨款将获得Sequel II平台，这是苏格兰的第一个平台，并将其嵌入爱丁堡基因组公司，使我们能够为生物和生物技术研究界提供低成本，高质量的长读测序服务。我们将利用这笔投资来支持那些希望为农业、生物技术、遗传学、免疫学和合成生物学等不同主题生成基因组数据的研究人员。

The genomics revolution is entering its third phase with the advent of technologies that can generate single molecule DNA sequencing data extending 100s of kilobases. Long DNA sequence reads can be used to generate high quality reference genomes, to better understand gene expression, and to sequence and sort DNA in complex mixtures such as environmental samples of soil or water. The Pacific Biosciences Sequel II System is a new long-read DNA sequencing platform that generates extremely large amounts of data at an affordable cost. A key feature of Sequel II is that it can generate highly accurate long reads, by reading a DNA molecule multiple times to correct errors. This grant will acquire the Sequel II platform, the first in Scotland, embed this within Edinburgh Genomics, and allow us to offer low-cost, high quality long read sequencing as a service to the biological and biotechnology research community. We will use the investment to support researchers who want to generate genomic data for diverse topics such as agriculture, biotechnology, genetics, immunology and synthetic biology.