DESIGN AND EVALUATION OF AN ARTIFICIAL SKIN

转录终止，就像转录启动一样，是一种高度 调控过程，在基因调控中发挥重要作用 表情。拟议的研究是为了彻底检查 噬菌体lambda N基因产物在大肠杆菌中的终止和抑制， 使用克隆、遗传学和生化方法的组合。 将确定RNA中N-识别元件的结构，并 涉及这种元素的可能的大分子相互作用将是 学习。 大肠杆菌NusB蛋白调控转录的假说 在特定终止子的终止将通过遗传和体外测试 实验。观察到大肠杆菌中S10蛋白的改变 NUSE突变体揭开了通常沉默的终结者的面具，将在 详细信息，以提供对NUS基因在 对终止的控制。 N蛋白改变RNA聚合酶形成末端的假说 将彻底检查抗性转录复合体。使用 转录-翻译耦合系统以及NUS因子的作用 由于核糖体参与他的过程的提议将是 调查过了。将从此分离N-修饰的转录复合体 系统和适当标记的组件将用于研究 N改变转录复合体的基础。这些研究将 提供基本信息以加深我们对该法规的了解 关于基因表达的。

Termination of transcription, like transcription initiation is a highly regulated process and plays a major role in the control of gene expression. The proposed research is to examine thoroughly the process of termination and its suppression in E. coli by phage lambda N gene product, using a combination of cloning, genetics and biochemical approaches. The structure of the N-recognition element in RNA will be determined, and the possible macromolecular interactions involving this element will be studied. The hypothesis that the E. coli NusB protein modulates transcription termination at specific terminators will be tested by genetic and in vitro experiments. The observation that the altered S10 protein in the E. coli nusE mutant unmasks a normally-silent terminator, will be investigated in detail to provide a complete understanding of the role of nus genes in control of termination. The hypothesis that N protein alters RNA polymerase to form a termination resistant transcription complex will be thoroughly examined. Using a coupled transcription-translation system, the role of Nus factors as well as the proposed involvement of ribosomes int his process will be investigated. N-modified transcription complex will be isolated from this system and appropriately labelled components will be used to study the basis of alteration of the transcription complex by N. These studies will provide fundamental information to further our knowledge of the regulation of gene expression.