PROTEIN LIPID INTERACTION

本提案的目的是研究几个定义明确的系统，在这些系统中 蛋白质和脂类相互作用。这种互动对于 生物膜的组装和功能。系统是i) 细菌酶环丙烷脂肪酸合成酶的作用(II) 含脂噬菌体PR4的形态发生及III)分离 酵母突变株，酿酒酵母，缺乏 磷脂交换蛋白。环丙烷脂肪酸合成酶是 只有已知的离散酶能与非极性部分发生特异性反应 磷脂双层的。我们建议准备大量的这种 利用重组DNA技术研究其与酶的相互作用 以及它的磷脂底物。噬菌体PR4的衣壳类物质来自 细菌膜和这一过程似乎是一个很好的研究模型。 通过遗传手段实现膜的形态发生。我们建议使用一个组合 来研究噬菌体产生的机制 脂类被结合在一起。脂交换蛋白在自然界中普遍存在。 并对一些哺乳动物蛋白质进行了纯化和研究。 然而，这些蛋白质的功能尚不清楚。酿酒酵母就有这样的 蛋白质，是一种典型的真核生物，具有复杂的遗传系统。 我们建议利用这种遗传系统来分离出不同于基因的突变体。 这些蛋白质。这样的突变体将允许推断这些基因的作用 真核细胞中的蛋白质。生物的正常功能和形成 膜对于细胞的存活是必不可少的。这些项目应该提供 关于膜形态发生机制的急需信息和 功能。大量的疾病过程与缺陷有关。 在膜形态发生中。

The aim of this proposal is to study several well-defined systems in which proteins and lipids interact. Such interaction are essential to the assembly and function of biological membranes. The systems are i) the action of the bacterial enzyme cyclopropane fatty acid synthase, ii) morphogenesis of the lipid-containing bacteriophage PR4, and iii) isolation of mutants of the yeast, Saccharomyces cereviseae, deficient in phospholipid exchange proteins. Cyclopropane fatty acid synthase is the only discrete enzyme known to specifically react with the nonpolar portion of a phospholipid bilayer. We propose to prepare large quantities of this enzyme by recombinant DNA techniques and to study its detailed interaction with its phospholipid substrate. Phage PR4 acquires its capsid lipid from the bacterial membrane and this process seems a good model to study membrane morphogenesis by genetic means. We propose to use a combination of genetics and biochemistry to study the mechanisms whereby the phage lipids are incorporated. Lipid exchange proteins are ubiquitous in nature and a number of mammalian proteins have been purified and studied. However, the function of these proteins is unknown. S. cereviseae has such proteins, is a typical eucaryote, and has a sophisticated genetic system. We propose to exploit this genetic system to isolate mutants difficient in these proteins. Such mutants will allow deduction of the role of these proteins in eucaryotic cells. Proper function and formation of biological membranes is essential for cell viability. These projects should provide much needed information on the mechanism of membrane morphogenesis and function. A large number of disease processes are associated with defects in membrane morphogenesis.