SPECIFIC GENE EXPRESSION IN EUCARYOTIC ORGANISMS

我们建议研究特定基因在细胞、染色质和 净化系统 这些包括以下定义的基因：人/大鼠胰岛素， 3个大鼠淀粉酶基因、肝炎病毒DNA（4个基因）（均由RNA转录 聚合酶III和酵母358核糖体RNA（RNA聚合酶I）和5S，tRNA 基因RNA聚合酶I。 胰岛素、淀粉酶、肝炎病毒基因表达将 在非洲爪蟾卵母细胞、转化/转染细胞（SV 40）中进行研究， 做/不表达基因。 DNA片段的测定和修饰 控制表达和定点整合到染色体中， 提出了 将通过以下方法分析表达/非表达细胞的染色质： DNA酶消化，染色质和提取物中基因的特异性表达 用于鉴定转录特异性因子。 RNA聚合酶I，RNA聚合酶转录因子 纯核糖体染色质（酵母）和提取物/酵母的简化 爪蟾 控制这些基因表达的因素的作用将是 研究了

We propose to study the expression of specific genes in cellular, chromatin and purified systems. These include the following defined genes: human/rat insulin, three rat amylase genes, hepatitis virus DNA (4 genes) (all transcribed by RNA polymerase III, and yeast 358 ribosomal RNA (RNA polymerase I) and 5S, tRNA genes RNA polymerase I. Insulin, amylase, hepatitis virus genes expression will be studied in Xenopus oocytes, in transformed/transfected cells (SV40) that do/do not express the genes. Determination and modification of DNA segments controlling expression and site-directed integration into chromosomes is proposed. Chromatin of expressing/non-expressing cells will be analyzed by DNAse digestion, specific expression of genes in chromatin and in extracts employed to identify transcription specific factors. RNA polymerase I, RNA polymerase transcription factors indentified by simplification of pure ribosomal chromatin (yeast) and from extracts/yeast Xenopus. Role of factors in controlling expression of these genes will be studied.